NDLI: Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L. using abiotic elicitors

Content Provider	Springer Nature Link
Author	Hashemi, Seyed Mohammad Naghavi, Mohammad Reza
Copyright Year	2015
Abstract	Nowadays, hairy root culture elicitation is an excellent platform with many advantages which make it an attractive system for using in plant biochemistry and molecular biology. Here, an efficient system for A. rhizogenes transformation in oriental poppy, Papaver orientale, was developed. Meanwhile, the expression level of various genes (COR, SalAT, SalR, T6ODM, CODM and Salsyn) in the morphinan pathway for response to methyl jasmonate (MJ) and salicylic acid (SA) at different time points (6, 12, 24 and 48 h) was examined. Afterwards, in vitro production of thebaine, morphine and codeine was investigated. The wild-type A. rhizogenes strain ATCC15834 was able to produce transformed roots with the highest frequency on the hypocotyl explant. Quantitative reverse-transcription PCR analysis showed that the expression of SalR, Salsyn, SalAT, T6ODM and CODM were significantly up-regulated with MJ treatment at 48 h. Moreover, the expression levels of Salsyn, T6ODM and CODM was up-regulated considerably in SA at 48 h treatment, while, the expression of SalR was down-regulated at 24 h. Furthermore, high-performance liquid chromatography analysis showed that MJ elicitation enhanced thebaine and morphine at 48 h by 2.63-fold (3.08 mg g$^{−1}$) and 6.18-fold (5.38 mg g$^{−1}$), respectively, and also codeine was increased at 24 h by 3.67-fold (2.57 mg g$^{−1}$). In addition, the accumulation of morphine and thebaine was increased by 4.22-fold (2.87 mg g$^{−1}$) and 2-fold (1.66 mg g$^{−1}$) in comparison with control treatment after SA treatment for 48 h. Our findings suggest that oriental poppy hairy roots can be used as a good source for producing commercial alkaloids.
Starting Page	31
Ending Page	41
Page Count	11
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	125
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-12-12
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Elicitation Gene expression Hairy root culture Morphinan alkaloids Papaver orientale L. Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Enhanced production of isoflavones by elicitation in hairy root cultures of Soybean

Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthus nasutus (L.) Kurz

A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum

Hairy root induction of Papaver somniferum var. album, a difficult-to-transform plant, by A. rhizogenes LBA 9402

Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors

Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ.

Influence of medium and elicitors on the production of cocaine, amino acids and phytohormones by Erythroxylum coca calli

Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L. using abiotic elicitors

Similar Documents

Enhanced production of isoflavones by elicitation in hairy root cultures of Soybean

Effect of plant growth regulators and elicitors on rhinacanthin accumulation in hairy root cultures of Rhinacanthus nasutus (L.) Kurz

A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum

Hairy root induction of Papaver somniferum var. album, a difficult-to-transform plant, by A. rhizogenes LBA 9402

Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

Elicitation of thiophene production by cultured hairy roots of Tagetes patula

Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors

Hairy root induction and polysaccharide production of medicinal plant Callerya speciosa Champ.

Influence of medium and elicitors on the production of cocaine, amino acids and phytohormones by Erythroxylum coca calli

Production and gene expression of morphinan alkaloids in hairy root culture of Papaver orientale L. using abiotic elicitors