NDLI: A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum

Content Provider	Springer Nature Link
Author	Sharafi, Ali Sohi, Haleh Hashemi Mousavi, Amir Azadi, Pejman Razavi, Khadijeh Ntui, Valentine Otang
Copyright Year	2012
Abstract	An efficient and reliable transformation system for a very important medicinal plant Papaver bracteatum was developed through optimization of several factors that affect the rate of effective A. rhizogenes-mediated transformation and growth rate of hairy root. Five bacterial strains, A4, ATCC15834, LBA9402, MSU440 and A13, and three explants types, hypocotyls, leaves and excised shoots were examined. The highest frequency of transformation was achieved using LBA9402 strain in the excised shoots. Several inoculation and co-cultivation media and different concentration of arginine were evaluated using LBA9402 strain and the excised shoots as explant. Interestingly, a drastic increase in the frequency of transformation (47.3 %) was observed when Murashige and Skoog medium containing 1 mM arginine and lacking NH$_{4}$NO$_{3}$ KH$_{2}$PO$_{4}$, KNO$_{3}$ and CaCl$_{2}$ was used. The effect of sucrose concentration and the ratio of NH$_{4}$ $^{+}$: NO$_{3}$ $^{−}$ on hairy root biomass was examined. Maximum biomass was obtained in 30 g/l sucrose and 20:10 mM ratio of NH$_{4}$ $^{+}$ to NO$_{3}$ $^{−}$ on MS medium. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and Southern hybridization.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	01676857
Journal	Plant Cell, Tissue and Organ Culture (PCTOC)
Volume Number	113
Issue Number	1
e-ISSN	15735044
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-10-19
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Agrobacterium rhizogenes Transformation Co-cultivation Macro elements Hairy root Papaver bracteatum Plant Sciences Plant Physiology Plant Genetics & Genomics Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Horticulture

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