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| Content Provider | Springer Nature Link |
|---|---|
| Author | Han, Y. Y. Ming, F. Wang, J. W. Wen, J. G. Ye, M. M. Shen, D. L. |
| Copyright Year | 2006 |
| Abstract | Chalcone synthase (CHS, EC 2.3.1.74) is the key enzyme involved in flavonoid and anthocyanin biosynthesis. A complete DNA sequence of chalcone synthase gene designated Pchs1 was isolated by means of usual and then inverse polymerase chain reactions from genomic DNA of an orchid, Phalaenopsis hybrida, cv. Formosa rose. Nucleotide sequence analysis based on alignment with published Phalaenopsis chs cDNA revealed that Pchs1 contained an intact open reading frame of 1173-bp with one 109-bp intron at the conserved site. The deduced polypeptide (PCHS1) from Pchs1 comprised 390 amino acids with a predicted mol wt of 42.5 kD. PCHS1 showed 61–65% identities with CHS from other plants and retained most of the conserved residues. Some putative cis-regulatory elements were present at the 5′ and 3′ flanking regions of Pchs1. Southern blot analysis predicted at least four chs-like genes, thus indicating the presence of a small multigene chs family in P. hybrida. Relative quantitative RT-PCR showed that Pchs1 is expressed in petals at early flower development as well as in lip tissue when the flower has just opened. |
| Starting Page | 223 |
| Ending Page | 230 |
| Page Count | 8 |
| File Format | |
| ISSN | 10214437 |
| Journal | Russian Journal of Plant Physiology |
| Volume Number | 53 |
| Issue Number | 2 |
| e-ISSN | 16083407 |
| Language | English |
| Publisher | Nauka/Interperiodica |
| Publisher Date | 2006-01-01 |
| Publisher Place | Moscow |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Phalaenopsis hybrida orchid chalcone synthase gene expression flanking region anthocyanins Plant Sciences Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science |
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