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| Content Provider | Springer Nature Link |
|---|---|
| Author | Han, Ying Ying Ming, Feng Wang, Jing Wen Ye, Ming Ming Shen, Da Leng |
| Copyright Year | 2005 |
| Abstract | A new complete DNA sequence of the chalcone synthase (CHS, EC2.3.1.74) gene, which is specially expressed in floral organs ofPhalaenopsis hybrida cv. Formosa rose, was cloned. The gene, designatedPchs, contained an open-reading frame of 1173 bp disrupted by one 103-bp intron at the conserved site. CHS protein predicted fromPchs showed 61%–65% identities with CHS of other plant species and retained most of the conserved residues. Some putativecis elements characteristic of the CHS gene were found at the promoter region ofPchs. Southern blot analysis indicated that CHS was encoded by a small multigene family in the genome ofPhalaenopsis. Relative quantitative reverse transcriptase-polymerase chain reaction showed thatPchs was expressed both in pigmented flower tissues, including petal, lip, and sepal, at early developmental stages and in colorless reproductive tissue at a late stage. Expression of the coding sequence ofPchs in tobacco can lead to darker flower limbs than seen in controls, and some transgenic plants exhibited abnormality in growth of pollen tubes. These data confirm the identity and function ofPchs and further suggest that overexpression of thePhalaenopsis CHS gene could be used to manipulate the color of flowers of other plants. |
| Starting Page | 193 |
| Ending Page | 194 |
| Page Count | 2 |
| File Format | |
| ISSN | 07359640 |
| Journal | Plant Molecular Biology Reporter |
| Volume Number | 23 |
| Issue Number | 2 |
| e-ISSN | 15729818 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2005-01-01 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | anthocyanin chalcone synthase expression Phalaenopsis hybrida transgenic tobacco Plant Sciences Plant Physiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Molecular Biology |
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