NDLI: Antiradical Capacity and Induction of Apoptosis on HeLa Cells by a Phaseolus vulgaris Extract

Content Provider	Springer Nature Link
Author	Aparicio Fernández, Xochitl Reyso Camacho, Rosalia Castaño Tostado, Eduardo García Gasca, Teresa González de Mejía, Elvira Guzmán Maldonado, S. Horacio Elizondo, Guillermo Yousef, Gad Gabra Lila, Mary Ann Loarca Pina, Guadalupe
Copyright Year	2007
Abstract	Jamapa bean is a black Phaseolus vulgaris variety rich in condensed tannins, anthocyanins and flavonols with interesting biological activities. The objective of this work was to evaluate the antiradical capacity (ARC) of a Jamapa bean methanolic extract (BME) and some of the proanthocyanidin-rich fractions derived from it, using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The effect of the BME on some proteins involved in apoptosis on HeLa cells was also evaluated. A strong correlation between proanthocyanidin concentration in BME and antiradical capacity was found, suggesting that these compounds contribute significantly to antiradical activity. BME was a better radical scavenger than butylated hydroxytoluene (45.6 and 33.9% ARC at 400 µM, respectively). Two proanthocyanidin-rich fractions obtained after a preliminary separation of the BME using Toyopearl (TP4 and TP6) exhibited a higher antiradical activity than the parent extract. The treatment of HeLa cells with 35 µg BME/ml/24 h increased the expression of Bax and Caspase-3, pro-apoptotic proteins (6.13 and 1.2 times for Caspase-3 and Bax, respectively). The mechanism of action of some proteins involved in apoptosis was also evaluated, and the results suggest that black Jamapa bean could be an important source of polyphenolic compounds with potential biological use as antioxidant and anticancer agents.
Starting Page	35
Ending Page	40
Page Count	6
File Format	PDF
ISSN	09219668
Journal	Plant Foods for Human Nutrition
Volume Number	63
Issue Number	1
e-ISSN	15739104
Language	English
Publisher	Springer US
Publisher Date	2007-12-19
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antiradical capacity Apoptosis HeLa cells Phaseolus vulgaris L. Phenolic compounds Plant Physiology Ecology Nutrition Chemistry/Food Science Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry Food Science

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