NDLI: Overexpression of Doritaenopsis Hybrid EARLY FLOWERING 4-like4 Gene, DhEFL4, Postpones Flowering in Transgenic Arabidopsis

Content Provider	Springer Nature Link
Author	Chen, Weiwei Qin, Qiaoping Zheng, Yongping Wang, Chun Wang, Shuo Zhou, Mingbing Zhang, Chi Cui, Yongyi
Copyright Year	2015
Abstract	The EARLY FLOWERING 4 (ELF4) gene has been reported as a requirement for photoperiod perception and circadian function. In this study, an ELF4 homolog gene EARLY FLOWERING 4-like4 (EFL4) was isolated from Doritaenopsis “Tinny Tender” (Doritaenopsis Happy Smile × Happy Valentine) and designated as DhEFL4. The acquired DhEFL4 cDNA was 593 bp with a 345 bp open reading frame encoding 114 amino acids. The putative DhEFL4 protein contained a conserved region, DUF1313, which is shared by the ELF4 family. Amino acid sequence alignment showed that DhEFL4 shared high similarity to ELF4 and ELF4-like homologs from other species. The DhEFL4 promoter was also obtained, and 241 cis-elements were found. The diurnal expression of DhEFL4, which may be affected by the EVENINGAT (EE) and circadian motif was analyzed by quantitative PCR under short- and long-day conditions. Reverse-transcription PCR analysis results showed that DhEFL4 is an evening-expressed circadian gene. DhEFL4 was expressed in both vegetative and reproductive organs, and its transcripts reached higher levels in the buds. To further study the function of the DhEFL4 gene, DhEFL4 was overexpressed in Arabidopsis thaliana by Agrobacterium-mediated transformation. The flowering of these transgenic plants was more delayed than that of wild-type Arabidopsis by 12−14 days, and flower stems increased. Results indicated that DhEFL4 may perform an important function in the regulation of flowering.
Starting Page	103
Ending Page	117
Page Count	15
File Format	PDF
ISSN	07359640
Journal	Plant Molecular Biology Reporter
Volume Number	34
Issue Number	1
e-ISSN	15729818
Language	English
Publisher	Springer US
Publisher Date	2015-06-25
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Doritaenopsis hybrid DhEFL4 ELF4 EFL4 Overexpression Promoter Plant Sciences Plant Breeding/Biotechnology Proteomics Metabolomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Plant Science Molecular Biology

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