NDLI: Transferability of Microsatellites from Psidium guajava to Eugenia, Myrciaria, Campomanesia, and Syzygium Species (Myrtaceae)

Content Provider	Springer Nature Link
Author	gueira, Angélica Maria Ferreira, Adésio Silva Ferreira, Marcia Flores
Copyright Year	2015
Abstract	Myrtaceae has many fruit-bearing species of economic and ecological relevance worldwide, but with microsatellite molecular markers (simple sequence repeats, SSR) available for only few of them. Thus, the transferability of SSR is advantageous for characterization, conservation, and breeding of these species. In this work, 158 SSR from Psidium guajava were evaluated for transferability in 18 fruit trees of the genera Eugenia, Campomanesia, Myrciaria, and Syzygium. Of those, 152 SSR transferred to at least 1 and up to 16 species; 47 SSR amplified in more than 10 species, the most conserved; and 13 SSR transferred to 1 or 2 species, demonstrating specificity. A large number of conserved SSR are also present in the genetic map of P. guajava, and may be helpful for synteny and comparative genomic studies. The average of transferability was lower for Syzygium and similar in the other genera. Transferability in the species varied from 20 SSR for Syzygium cumini to 91 for Myrciaria sp.2, being higher on average in the genus Eugenia. Clustering based on the pattern of transferability between species revealed two groups, one composed by Eugenia and Campomanesia and another by Syzygium and Myrciaria, which indicates conserved microsatellite regions within and between genera. This study also allowed selection of the most suitable SSR for each species and genus by amplification quality and study type, thus contributing for a better comprehension of the relationships between the species, taxonomy, structure and genetic diversity, construction of genetic maps, and breeding and conservation programs in the family.
Starting Page	249
Ending Page	256
Page Count	8
File Format	PDF
ISSN	07359640
Journal	Plant Molecular Biology Reporter
Volume Number	34
Issue Number	1
e-ISSN	15729818
Language	English
Publisher	Springer US
Publisher Date	2015-07-09
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	SSR Genetic diversity Genetic mapping Conservation Plant Sciences Plant Breeding/Biotechnology Proteomics Metabolomics Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Plant Science Molecular Biology

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