NDLI: Arsenic methylation by a genetically engineered Rhizobium-legume symbiont

Content Provider	Springer Nature Link
Author	Zhang, Jun Xu, Yan Cao, Tingting Chen, Jian Rosen, Barry P. Zhao, Fang Jie
Copyright Year	2017
Abstract	Arsenic (As) is one of the most widespread environmental contaminants. The aim of our study was to test a novel bioremediation system based on the symbiosis between leguminous plant and genetically engineered rhizobia.The arsenite [As(III)] S-adenosylmethionine methyltransferase gene (CrarsM) from the alga Chlamydomonas reinhardtii was inserted into the chromosome of Rhizobium leguminosarum bv. trifolii strain R3. The As methylation ability of the recombinant Rhizobium was tested under free living conditions and in symbiosis with red clover plants. Arsenic speciation was determined using high-performance liquid chromatography-inductively coupled plasma mass spectrometry.Under free-living conditions, CrarsM-recombinant R. leguminosarum gained the ability to methylate As(III) to methylated arsenicals, including methylarsenate [MAs(V)], dimethylarsenate [DMAs(V)] and trimethylarsine oxide [TMAs(V)O]. Red clover plants were inoculated with either control (non-recombinant) or CrarsM-recombinant R. leguminosarum and exposed to 5 or 10 μM arsenite. No methylated As species were detected in red clover plants inoculated with control R. leguminosarum. In contrast, all three methylated species were detected in both the nodules and the shoots when the recombinant Rhizobium established symbiosis with red clover, accounting for 74.7–75.1% and 29.1–42.4% of the total As in the two plant tissues, respectively. The recombinant symbiont also volatilized small amounts of As.The present study demonstrates that engineered rhizobia expressing an algal arsM gene can methylate and volatilize As, providing a proof of concept for potential future use of legume-rhizobia symbionts for As bioremediation.
Starting Page	259
Ending Page	269
Page Count	11
File Format	PDF
ISSN	0032079X
Journal	Plant and Soil
Volume Number	416
Issue Number	1-2
e-ISSN	15735036
Language	English
Publisher	Springer International Publishing
Publisher Date	2017-02-27
Publisher Place	Cham
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arsenic Arsenite S-adenosylmethionine methyltransferase Arsenic methylation Bioremediation Rhizobia Symbiosis Plant Sciences Soil Science & Conservation Plant Physiology Ecology
Content Type	Text
Resource Type	Article
Subject	Soil Science Plant Science

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