NDLI: Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

Content Provider	Springer Nature Link
Author	Lee, Mi Hyun Kim, Bohye Song, Sang Kee Heo, Jung Ok Yu, Nan Ie Lee, Shin Ae Kim, Miran Kim, Dong Gwan Sohn, Sung Oh Lim, Chae Eun Chang, Kwang Suk Lee, Myeong Min Lim, Jun
Copyright Year	2008
Abstract	GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a “phenome-ready unimutant collection” of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein–protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein–protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.
Starting Page	659
Ending Page	670
Page Count	12
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	67
Issue Number	6
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-26
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arabidopsis GRAS family Phenome-ready unimutant collection SCARECROW-LIKE23 Yeast two-hybrid assay Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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