NDLI: Barley (Hordeum vulgare L.) inositol monophosphatase: gene structure and enzyme characteristics

Content Provider	Springer Nature Link
Author	Fu, Jianming Peterson, Kevin Guttieri, Mary Souza, Edward Raboy, Victor
Copyright Year	2008
Abstract	The cellular myo-inositol (Ins) pool is important to many metabolic and signaling pathways in plants. Ins monophosphatase (IMPase; EC 3.1.3.25) activity is essential for the de novo synthesis of myo-Inositol (Ins), and for recycling of Ins in Ins(1,4,5)P$_{3}$. However, proteins encoded by at least one family of IMP genes also have l-galactose-1-P phosphatase activity important to ascorbic acid synthesis, indicating a bifunctionality that links these two branches of carbon metabolism. As part of research into the regulation of Ins synthesis and supply during seed development, the barley IMP-1 gene and gene products were studied. The 1.4 kb barley IMP-1 promoter contains one low temperature response element (RE), two heat shock REs, one gibberellin and two auxin REs, and five sugar REs. Barley IMP-1 is expressed in all tissues assayed, and expression levels were not greatly altered by abiotic stress treatments. Reduced use of Ins for Ins P$_{6}$ synthesis in developing seed of barley low phytic acid (lpa) mutants results in Ins accumulation, and IMP-1 expression is reduced in proportion to the increase in Ins level. The barley recombinant enzyme had a lower K$_{m}$, indicating higher affinity, for d/l-Ins(3)P$_{1}$ (K$_{m}$ = 9.7 μM) as compared with reported K$_{m}$ (Ins P$_{1}$) values for other eukaryotic IMPases (43–330 μM) or with a reported K$_{m}$ (l-Gal-1P) of 150 μM for a kiwifruit (Actinidia deliciosa) enzyme. These and other data indicate that the barley IMP-1 gene is regulated at least in part in response to Ins metabolic needs, and that the enzyme it encodes displays catalytic properties well suited for a role in Ins synthesis, in addition to other roles as an l-gal-1-P phosphatase important to ascorbate synthesis, or as an IMPase important to Ins(1,4,5)P$_{3}$ signal recycling.
Starting Page	629
Ending Page	642
Page Count	14
File Format	PDF
ISSN	01674412
Journal	Plant Molecular Biology
Volume Number	67
Issue Number	6
e-ISSN	15735028
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Inositol Phytic acid Seed development Plant Pathology Biochemistry Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Medicine Agronomy and Crop Science

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