NDLI: Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Please wait, while we are loading the content...

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Development of models for estimating leaf chlorophyll and nitrogen contents in tree species with respect to seasonal changes

Influence of foliar-applied triacontanol on growth, gas exchange characteristics, and chlorophyll fluorescence at different growth stages in wheat under saline conditions

Gender, mediterranean drought, and seasonality: photosystem II photochemistry in Pistacia lentiscus L.

Effect of chromate on photosynthesis in Cr(VI)-resistant Chlorella

Effects of drought stress on the evergreen Quercus ilex L., the deciduous Q. robur L. and their hybrid Q. × turneri Willd.

Role of cyanide-resistant respiration during light-induced attraction of predators to herbivore-infested leaves

Effect of nitrogen application and elevated CO$_{2}$ on photosynthetic gas exchange and electron transport in wheat leaves

Effects of water stress and fertilization on leaf gas exchange and photosynthetic light-response curves of Bothriochloa ischaemum L.

Leaf area estimation in muskmelon by allometry

Salinity response pattern and isolation of catalase gene from halophyte plant Aeluropus littoralis

Effect of high temperature on dehydration-induced alterations in photosynthetic characteristics of the resurrection plant Haberlea rhodopensis

Photosynthetica : Volume 51, Issue 3, September 2013

Photosynthetica : Volume 51, Issue 2, June 2013

Photosynthetica : Volume 51, Issue 1, March 2013

Photosynthetica : Volume 50

Photosynthetica : Volume 49

Photosynthetica : Volume 48

Photosynthetica : Volume 47

Photosynthetica : Volume 46

Photosynthetica : Volume 45

Photosynthetica : Volume 44

Photosynthetica : Volume 43

Photosynthetica : Volume 42

Photosynthetica : Volume 41

Photosynthetica : Volume 40

Photosynthetica : Volume 39

Photosynthetica : Volume 38

Photosynthetica : Volume 37

Photosynthetica : Volume 36

Photosynthetica : Volume 35

Photosynthetica : Volume 34

Photosynthetica : Volume 33

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Content Provider	Springer Nature Link
Author	Šetlíková, E. Sofrová, D. Kovář, V. Budáč, P.
Copyright Year	2013
Abstract	Photosystem (PS) II particles retaining a high rate of O$_{2}$ evolution were isolated from the mesophilic filamentous cyanobacterium, Spirulina platensis. To achieve high production of PSII complexes in the cells, irradiance from halogen incandescent lamps was used. Disruption of cells by vibration of glass beads proved to be the most suitable procedure for isolation of thylakoid membranes. The selectivity of detergents for PSII particle preparation rose in the order of Triton X-100 < decyl-β-D-glucopyranoside < dodecyldimethyl-aminooxide < n-heptyl-β-D-thioglucoside < N-dodecyl-N,N-dimethylammonio-3-propane sulphonate < n-octyl-β-thioglycoside < octylglucoside < n-dodecyl-β-D-maltoside. The last four detergents yielded extracts, from which pure PSII particles not contaminated by PSI complexes could be obtained by sucrose-gradient centrifugation (20–45%) at the 43% sucrose level. We assumed both the acceptor and donor sides of the isolated n-dodecyl-β-D-maltoside (DM) particles to be intact due to high oxygen production by DM particles [1,500 meq(e$^{−}$) mol$^{−1}$ (Chl) s$^{−1}$] achieved in the presence of all artificial acceptors tested. The PSII particle fraction from the sucrose gradient was used with immobilized metal (Cu$^{2+}$) affinity chromatography (IMAC) for the preparation of the PSII core complex. By washing the column with a MES buffer containing MgCl$_{2}$ and CaCl$_{2}$, the phycobiliproteins were stripped off. The PSII core complex was eluted in a buffer containing 1% DM, mannitol, MgCl$_{2}$, NaCl, CaCl$_{2}$, and ɛ-aminocaproic acid. SDS-PAGE of the core complex provided pure bands of D1 and D2 proteins and PsbO protein from thylakoid membrane, which were used to raise polyclonal antibodies in rabbits. These antibodies recognized D1 and D2 not only as monomers of 31 and 32 kDa proteins, but also as heterodimers of D1, D2 corresponding to the band of 66 kDa on SDS-PAGE. This was in contrast to antibodies of synthetic determinants, which reacted only with the monomers of D1 and D2 proteins. These negative reactions against heterodimers of D1, D2 supported the hypothesis that dimeric forms of PSII reaction centre proteins have a C-terminal sequence sterically protected against a reaction with specific antibodies.
Starting Page	517
Ending Page	530
Page Count	14
File Format	PDF
ISSN	03003604
Journal	Photosynthetica
Volume Number	51
Issue Number	4
e-ISSN	15739058
Language	English
Publisher	The Institute of Experimental Biology of the Czech Academy of Sciences
Publisher Date	2016-06-03
Publisher Place	Praha
Access Restriction	Subscribed
Subject Keyword	Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Isolation and Characterization of an Oxygen Evolving Photosystem 2 Core Complex from the Thermophilic Cyanobacterium Mastigocladus laminosus

Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea

Refined purification and further characterization of oxygen-evolving and Tris-treated Photosystem II particles from the thermophilic Cyanobacterium synechococcus sp.

Isolation and characterization of thylakoid membranes from the filamentous cyanobacterium Nostoc punctiforme.

Salt stress induces a decrease in excitation energy transfer from phycobilisomes to photosystem II but an increase to photosystem I in the cyanobacterium Spirulina platensis.

A Highly Active Oxygen-Evolving Photosystem II Preparation from the Cyanobacterium Anacystis nidulans 1

Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoceros gracilis.

Simple method for isolation of oxygen evolving photosystem 2 core complex free of cytochrome f contamination

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Similar Documents

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

Isolation and Characterization of an Oxygen Evolving Photosystem 2 Core Complex from the Thermophilic Cyanobacterium Mastigocladus laminosus

Isolation and characterization of the oxygen-evolving photosystem II complex from the economical red alga Bangia fusco-purpurea

Refined purification and further characterization of oxygen-evolving and Tris-treated Photosystem II particles from the thermophilic Cyanobacterium synechococcus sp.

Isolation and characterization of thylakoid membranes from the filamentous cyanobacterium Nostoc punctiforme.

Salt stress induces a decrease in excitation energy transfer from phycobilisomes to photosystem II but an increase to photosystem I in the cyanobacterium Spirulina platensis.

A Highly Active Oxygen-Evolving Photosystem II Preparation from the Cyanobacterium Anacystis nidulans 1

Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoceros gracilis.

Simple method for isolation of oxygen evolving photosystem 2 core complex free of cytochrome f contamination

Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis