NDLI: Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoceros gracilis.

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Isolation and characterization of oxygen-evolving thylakoid membranes and photosystem II particles from a marine diatom Chaetoceros gracilis.

Content Provider	Semantic Scholar
Author	Nagao, Ryo Ishii, Akiko Tada, Osamu Suzuki, Takehiro Dohmae, Naoshi Okumura, Akinori Iwai, Masako Takahashi, Takeshi Kashino, Yasuhiro Enami
Copyright Year	2007
Abstract	Thylakoid membranes retaining high oxygen-evolving activity (about 250 micromol O(2)/mg Chl/h) were prepared from a marine centric diatom, Chaetoceros gracilis, after disruption of the cells by freeze-thawing. We also s쳮ded in purification of Photosystem II (PSII) particles by differential centrifugation of the thylakoid membranes after treatment with 1% Triton X-100. The diatom PSII particles showed an oxygen-evolving activity of 850 and 1045 micromol O(2)/mg Chl/h in the absence and presence of CaCl(2), respectively. The PSII particles contained fucoxanthin chlorophyll a/c-binding proteins in addition to main intrinsic proteins of CP47, CP43, D2, D1, cytochrome b559, and the antenna size was estimated to be 229 Chl a per 2 molecules of pheophytin. Five extrinsic proteins were stoichiometrically released from the diatom PSII particles by alkaline Tris-treatment. Among these five extrinsic proteins, four proteins were red algal-type extrinsic proteins, namely, PsbO, PsbQ', PsbV and PsbU, whereas the other one was a novel, hypothetical protein. This is the first report on isolation and characterization of diatom PSII particles that are highly active in oxygen evolution and retain the full set of extrinsic proteins including an unknown protein.
File Format	PDF HTM / HTML
DOI	10.1016/j.bbabio.2007.10.007
PubMed reference number	17996191
Journal	Medline
Volume Number	1767
Issue Number	12
Alternate Webpage(s)	https://core.ac.uk/download/pdf/82344940.pdf
Journal	Biochimica et biophysica acta
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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