NDLI: Guanidine Transport in a Human Choriocarcinoma Cell Line (JAR)

Content Provider	Springer Nature Link
Author	Zevin, Shoshana Schaner, Marci E. Illsley, Nicholas P. Giacomini, Kathleen M.
Copyright Year	1997
Abstract	Purpose. Many endogenous substances and xenobiotics are organic cations. Transplacental transport of organic cations is an important determinant of the delivery of these compounds to the fetus. The aim of this study was to determine the mechanisms of organic cation transport using the human choriocarcinoma cell line (JAR) as a model system with [$^{14}$C]guanidine as a ligand. Methods. Uptake studies of [$^{l4}$C]guanidine were carried out in JAR cell monolayers on day 2 after plating. Results. [$^{14}$C]guanidine uptake was temperature dependent, saturable (K$_{m}$ = 167 μM) and inhibited by many organic cations including amiloride, cimetidine, quinine, quinidine and nicotine. [$^{l4}$C]guanidine uptake exhibited a counterflux phenomenon indicative of a carrier-mediated process. The uptake of [$^{14}$C]guanidine was sodium and pH-independent and could be driven by an inside-negative membrane potential difference. Conclusions. This is the first demonstration of an electrogenic guanidine transporter in a human cell culture model. This transporter may play a role in the transplacental transport of many clinically used drugs and xenobiotics.
Starting Page	401
Ending Page	405
Page Count	5
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	14
Issue Number	4
e-ISSN	1573904X
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pharmacology/Toxicology Pharmacy Biochemistry Medical Law Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

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