NDLI: Activation of Antigen-Specific CD8$^{+}$ T Cells by Poly-DL-Lactide/Glycolide (PLGA) Nanoparticle-Primed Gr-1$^{high}$ Cells

Content Provider	Springer Nature Link
Author	Luo, Wen Hui Yang, Ya Wun
Copyright Year	2015
Abstract	The aim of this study was to investigate the induction of antigen-specific T cell activation and cell cycle modulation by a poly-DL-lactide/glycolide (PLGA) nanoparticle (NP)-primed CD11b$^{+}$Gr-1$^{high}$ subset isolated from mouse bone marrow.PLGA NPs containing the ovalbumin (OVA) antigen were prepared using the double emulsion and solvent evaporation method, and protein release rate and cell viability were determined. The Lin2$^{¯}$CD11b$^{+}$Gr-1$^{high}$Ly6c$^{low}$ (Gr-1$^{high}$) subset was sorted from the bone marrow of C57BL/6 J mice by fluorescence-activated cell sorting (FACS) and co-cultured with OT-I CD8$^{+}$ splenic T cells. Proliferation of OT-I CD8$^{+}$ T cells was monitored, and cell cycles were determined by 5-bromo-2′-deoxyuridine (BrdU) labeling.Treatment of Gr-1$^{high}$ cells with PLGA/OVA NPs upregulated expression of the SIINFEKL-H2K$^{b}$ complex in the context of MHC I. Co-cultures of OT-I CD8$^{+}$ T cells with the PLGA/OVA NP-primed Gr-1$^{high}$ cells induced the proliferation of T cells in vitro and modulated cell division and morphology. Treatment of Gr-1$^{high}$ cells with PLGA/OVA NPs also induced cell apoptosis and necrosis.This study demonstrated the function of PLGA/OVA NPs in the activation of OT-I CD8$^{+}$ T cells and the capability of cross-presentation via the Gr-1$^{high}$ polymorphonuclear subset from mouse bone marrow.
Starting Page	942
Ending Page	955
Page Count	14
File Format	PDF
ISSN	07248741
Journal	Pharmaceutical Research
Volume Number	33
Issue Number	4
e-ISSN	1573904X
Language	English
Publisher	Springer US
Publisher Date	2015-12-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pharmacology/Toxicology Pharmacy Biochemistry Medical Law Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Pharmacology Molecular Medicine Pharmacology (medical) Biotechnology Pharmaceutical Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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