NDLI: Glutamate-Dependent Translational Control Through Ribosomal Protein S6 Phosphorylation in Cultured Bergmann Glial Cells

Content Provider	Springer Nature Link
Author	Flores Méndez, Marco Escalante López, Miguel Martínez Lozada, Zila Hernández Kelly, Luisa C. Najimi, Mustapha Sokal, Etienne Ortega, Arturo
Copyright Year	2015
Abstract	Glutamate (Glu) the main excitatory neurotransmitter of the central nervous system regulates gene expression at different levels through the activation of specific membrane receptors and transporters expressed in neurons and glia cells. A membrane to nucleus signaling cascade triggered by this neurotransmitter has been described in cultured cerebellar Bergmann glia cells isolated from chick embryos. Furthermore, it has also been described that Glu receptors activation is linked to a modulation of [$^{35}$S]-methionine incorporation into newly synthesized polypeptides. In order to gain insight into the signal transduction cascades that participate in this effect, in the present study we characterized the phosphorylation of a critical component of the translational machinery, namely the ribosomal protein S6. The phosphorylation sites in rpS6 have been mapped to five clustered residues, Ser235, Ser236, Ser240, Ser244 and Ser247. Nevertheless, Ser236 phosphorylation is the primary phosphorylation site. The kinases responsible of this modification are p70$^{S6K}$ and p90$^{RSK}$. rpS6 phosphorylation increases the affinity of 40s subunit for mRNAs and thus facilitates translational initiation. Glutamate exposure of cultured cerebellar Bergmann glia cells results in a time- and dose-dependent increase in rpS6 phosphorylation. This effect is mainly observed at cytoplasm, and involves the phosphoinositol-3 kinase/protein kinase B pathway. Our results favor the notion of a continuous neuronal signaling to glia cells that regulates the proteome of these cells not only at the transcriptional level but also at the level of protein synthesis.
Starting Page	915
Ending Page	923
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	40
Issue Number	5
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2015-03-04
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ribosomal protein S6 Glutamate receptors Translational control Bergmann glia Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Glutamate-Dependent Translational Control in Cultured Bergmann Glia Cells: eIF2α Phosphorylation

Glutamate-Dependent BMAL1 Regulation in Cultured Bergmann Glia Cells

Methylphenidate Increases Glutamate Uptake in Bergmann Glial Cells

Glutamate Activates Protein Kinase B (PKB/Akt) through AMPA Receptors in Cultured Bergmann Glia Cells

An Acute Glutamate Exposure Induces Long-Term Down Regulation of GLAST/EAAT1 Uptake Activity in Cultured Bergmann Glia Cells

Glutamate-dependent transcriptional control in Bergmann glia: Sox10 as a repressor.

Glutamate-Dependent Transcriptional Regulation in Bergmann Glia Cells: Involvement of p38 MAP Kinase

Glutamate Regulates Dystrophin-71 levels in Glia Cells

Glutamate Receptor Stimulation Up-Regulates Glutamate Uptake in Human Müller Glia Cells

Glutamate-Dependent Translational Control Through Ribosomal Protein S6 Phosphorylation in Cultured Bergmann Glial Cells

Similar Documents

Glutamate-Dependent Translational Control in Cultured Bergmann Glia Cells: eIF2α Phosphorylation

Glutamate-Dependent BMAL1 Regulation in Cultured Bergmann Glia Cells

Methylphenidate Increases Glutamate Uptake in Bergmann Glial Cells

Glutamate Activates Protein Kinase B (PKB/Akt) through AMPA Receptors in Cultured Bergmann Glia Cells

An Acute Glutamate Exposure Induces Long-Term Down Regulation of GLAST/EAAT1 Uptake Activity in Cultured Bergmann Glia Cells

Glutamate-dependent transcriptional control in Bergmann glia: Sox10 as a repressor.

Glutamate-Dependent Transcriptional Regulation in Bergmann Glia Cells: Involvement of p38 MAP Kinase

Glutamate Regulates Dystrophin-71 levels in Glia Cells

Glutamate Receptor Stimulation Up-Regulates Glutamate Uptake in Human Müller Glia Cells

Glutamate-Dependent Translational Control Through Ribosomal Protein S6 Phosphorylation in Cultured Bergmann Glial Cells