NDLI: Physiopathological Effects of the NO Donor 3-Morpholinosydnonimine on Rat Cortical Synaptosomes

Content Provider	Springer Nature Link
Author	Blanco Garcia, J. Aldinucci, C. Maiorca, S. M. Palmi, M. Valoti, M. Buocore, G. Pessina, G. P.
Copyright Year	2008
Abstract	The NO donor 3-Morpholinosydnonimine (SIN-1) releases NO in the presence of molecular oxygen. In this study, we evaluated the effect of SIN-1 on mitochondria of rat cortical synaptosomes. We demonstrated in vitro that the amount of ONOO$^{−}$ generated and H$_{2}$O$_{2}$ formation directly correlated with SIN-1 concentration. The mean oxygen consumption by synaptosomal mitochondria was approximately 3.8 nmol of O$_{2}$ min$^{−1}$ mg$^{−1}$ protein, which decreased significantly in the presence of SIN-1 1 mM to 2.5 nmol O$_{2}$ min$^{−1}$ mg$^{−1}$. This decrease was not modified by catalase or Trolox, demonstrating that ONOO$^{−}$ was responsible for the effect. The same concentration of SIN-1 caused a significant decrease of ATP production by synaptosomal mitochondria and depolarized the mitochondrial membrane. Moreover, ROS production increased progressively and was completely inhibited by pre-incubation of synaptosomes with Trolox. Finally, phosphatidylserine was externalized and, at the same time, intrasynaptosomal lactate dehydrogenase decreased confirming both, the external membrane breakdown after the addition of SIN-1 and the damage to the synaptosomes.
Starting Page	931
Ending Page	941
Page Count	11
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	34
Issue Number	5
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2008-10-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nitric oxide NO donors Synaptosomes Mitochondria Neurology Biochemistry Neurosciences
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effect of Nitric Oxide Donors on GABA Uptake by Rat Brain Synaptosomes

The Effect of Mild and Severe Hypoxia on Rat Cortical Synaptosomes

Interaction of Nitric Oxide Donors and Ascorbic Acid on D-[$^{3}$H]Aspartate Efflux from Rat Striatal Slices

Effects of Linalool on Glutamate Release and Uptake in Mouse Cortical Synaptosomes

Anoxia-Induced Changes in Pyridine Nucleotide Redox State in Cortical Neurons and Astrocytes

Influence of Glucose Deprivation on Membrane Potentials of Plasma Membranes, Mitochondria and Synaptic Vesicles in Rat Brain Synaptosomes

Hypoxia-Induced Caspase-3 Activation and DNA Fragmentation in Cortical Neurons of Newborn Piglets: Role of Nitric Oxide

Nitric Oxide Production in Striatum and Pallidum of Cirrhotic Rats

Gas Phase Oxidants of Cigarette Smoke Increase Nitric Oxide Synthase and Xanthine Oxidase Activities of Rabbit Brain Synaptosomes

Physiopathological Effects of the NO Donor 3-Morpholinosydnonimine on Rat Cortical Synaptosomes

Similar Documents

Effect of Nitric Oxide Donors on GABA Uptake by Rat Brain Synaptosomes

The Effect of Mild and Severe Hypoxia on Rat Cortical Synaptosomes

Interaction of Nitric Oxide Donors and Ascorbic Acid on D-[$^{3}$H]Aspartate Efflux from Rat Striatal Slices

Effects of Linalool on Glutamate Release and Uptake in Mouse Cortical Synaptosomes

Anoxia-Induced Changes in Pyridine Nucleotide Redox State in Cortical Neurons and Astrocytes

Influence of Glucose Deprivation on Membrane Potentials of Plasma Membranes, Mitochondria and Synaptic Vesicles in Rat Brain Synaptosomes

Hypoxia-Induced Caspase-3 Activation and DNA Fragmentation in Cortical Neurons of Newborn Piglets: Role of Nitric Oxide

Nitric Oxide Production in Striatum and Pallidum of Cirrhotic Rats

Gas Phase Oxidants of Cigarette Smoke Increase Nitric Oxide Synthase and Xanthine Oxidase Activities of Rabbit Brain Synaptosomes

Physiopathological Effects of the NO Donor 3-Morpholinosydnonimine on Rat Cortical Synaptosomes