NDLI: Role of 1,25-Dihydroxyvitamin D$_{3}$ and Extracellular Calcium in the Regulation of Proliferation in Cultured SH-SY5Y Human Neuroblastoma Cells

Please wait, while we are loading the content...

Energy Metabolism of Synaptosomal Subpopulations from Different Neuronal Systems of Rat Hippocampus: Effect of L-Acetylcarnitine Administration In Vivo

Alteration of Oxygen Consumption and Energy Metabolism During Repetitive Exposure of Mice to Hypoxia

Nitric Oxide Modulates Muscarinic Acetylcholine Receptor Binding in the Cerebral Cortex of Gerbils

Tubulin and Glial Fibrillary Acidic Protein Gene Expression in Developing Fetal Human Brain at Midgestation

Regulation of Glial Na$^{+}$/K$^{+}$-ATPase by Serotonin: Identification of Participating Receptors

Clozapine's Antipsychotic Effects do not Depend on Blockade of 5-HT$_{3}$ Receptors

Activation of cPLA$_{2}$, PKC, and ERKs in the Rat Cerebral Cortex During Ischemia/Reperfusion

Cerebral Blood Flow Threshold and Regional Heterogeneity of Heat Shock Protein 72 Induction Following Transient Forebrain Ischemia in Rats

Ceramide Induces Apoptosis to Immature Cerebellar Granule Cells in Culture

Role of 1,25-Dihydroxyvitamin D$_{3}$ and Extracellular Calcium in the Regulation of Proliferation in Cultured SH-SY5Y Human Neuroblastoma Cells

3β [N-(N′, N′-Dimethylaminoethane)-Carbamoyl] Cholesterol (DC-Chol)-Mediated Gene Delivery to Primary Rat Neurons: Characterization and Mechanism

Decrease in Spontaneous Motor Activity and in Brain Lipid Peroxidation in Manganese and Melatonin Treated Mice

GFAPβ mRNA Expression in the Normal Rat Brain and after Neuronal Injury

Neurochemical Research : Volume 24, Issue 4, April 1999

Neurochemical Research : Volume 24, Issue 3, March 1999

Neurochemical Research : Volume 24, Issue 2, February 1999

Neurochemical Research : Volume 24, Issue 1, January 1999

Neurochemical Research : Volume 23

Neurochemical Research : Volume 22

Role of 1,25-Dihydroxyvitamin D$_{3}$ and Extracellular Calcium in the Regulation of Proliferation in Cultured SH-SY5Y Human Neuroblastoma Cells

Content Provider	Springer Nature Link
Author	Celli, Alessandra Treves, Cristina Nassi, Paolo Stio, Maria
Copyright Year	1999
Abstract	This study examines the effects of 1,25-dihydroxyvitamin D$_{3}$ [1,25(OH)$_{2}$D$_{3}$] on SH-SY5Y human neuroblastoma cells cultured in the presence of medium containing varying concentrations of calcium (0.1, 0.9, 1.4, 1.8 mM). Pyruvate kinase activity was assayed in SH-SY5Y cells incubated in variable calcium medium with or without 1, 10 or 100 nM 1,25(OH)$_{2}$D$_{3}$ for 48 h. The enzyme levels showed a significant increase in comparison with control, when the cells were incubated with 100 nM hormone in the presence of 0.1 mM calcium, while pyruvate kinase activity decreased, when the cells were treated with 100 nM 1,25(OH)$_{2}$D$_{3}$ in the presence of 1.8 mM calcium. The proliferative activity of SH-SY5Y was dependent on the extracellular concentration of calcium, being the highest at 1.8 mM calcium and completely absent at 0.1 mM calcium. In the presence of 1,25(OH)$_{2}$D$_{3}$, at the three concentrations used and after 48 h incubation, a significant decrease in cell number was always observed, without a direct correlation between 1,25(OH)$_{2}$D$_{3}$ effect and calcium concentration in the medium. [$^{3}$H]Thymidine incorporation in SH-SY5Y cells significantly increased in comparison with control, when the 48 h incubation with 1, 10 or 100 nM 1,25(OH)$_{2}$D$_{3}$ was carried out in the presence of 0.1 mM calcium, while, at the other calcium concentrations, the hormone did not cause any significant change in this parameter. The treatment of SH-SY5Y cells with 1 nM 1,25(OH)$_{2}$D$_{3}$ for 48 h did not affect cell morphology, when 0.1 mM calcium was present, while, in the medium containing 1.8 mM calcium, the treated cells showed a slight trend to differentiation. The differentiating effect of 10 μM all-trans retinoic acid, even if incomplete after 48 h treatment, was only observed in the cultures grown in 1.8 mM calcium, in comparison with those maintained in 0.1 mM calcium.
Starting Page	691
Ending Page	698
Page Count	8
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	24
Issue Number	5
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Neuroprotective Effects of Ugonin K on Hydrogen Peroxide-Induced Cell Death in Human Neuroblastoma SH-SY5Y Cells

Contribution of GPR30 for 1,25 dihydroxyvitamin D$_{3}$ protection in EAE

Potentiation of Carbachol-Induced Ca2+ Release by Peroxynitrite in Human Neuroblastoma SH-SY5Y Cells

Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells

Involvement of Matrix Metalloproteinases on the Inhibition of Cells Invasion and Migration by Emodin in Human Neuroblastoma SH-SY5Y Cells

Mitochondrial Dysfunction Enhances Susceptibility to Oxidative Stress by Down-Regulation of Thioredoxin in Human Neuroblastoma Cells

Kinetics of a Novel Isoform of Phosphate Activated Glutaminase (PAG) in SH-SY5Y Neuroblastoma Cells

Ascorbate Transport and Recycling by SH-SY5Y Neuroblastoma Cells: Response to Glutamate Toxicity

Kainic Acid Down-regulates NOP Receptor Density and Gene Expression in Human Neuroblastoma SH-SY5Y Cells

Role of 1,25-Dihydroxyvitamin D$_{3}$ and Extracellular Calcium in the Regulation of Proliferation in Cultured SH-SY5Y Human Neuroblastoma Cells

Similar Documents

Neuroprotective Effects of Ugonin K on Hydrogen Peroxide-Induced Cell Death in Human Neuroblastoma SH-SY5Y Cells

Contribution of GPR30 for 1,25 dihydroxyvitamin D$_{3}$ protection in EAE

Potentiation of Carbachol-Induced Ca2+ Release by Peroxynitrite in Human Neuroblastoma SH-SY5Y Cells

Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells

Involvement of Matrix Metalloproteinases on the Inhibition of Cells Invasion and Migration by Emodin in Human Neuroblastoma SH-SY5Y Cells

Mitochondrial Dysfunction Enhances Susceptibility to Oxidative Stress by Down-Regulation of Thioredoxin in Human Neuroblastoma Cells

Kinetics of a Novel Isoform of Phosphate Activated Glutaminase (PAG) in SH-SY5Y Neuroblastoma Cells

Ascorbate Transport and Recycling by SH-SY5Y Neuroblastoma Cells: Response to Glutamate Toxicity

Kainic Acid Down-regulates NOP Receptor Density and Gene Expression in Human Neuroblastoma SH-SY5Y Cells

Role of 1,25-Dihydroxyvitamin D$_{3}$ and Extracellular Calcium in the Regulation of Proliferation in Cultured SH-SY5Y Human Neuroblastoma Cells