NDLI: CoCl$_{2}$-Induced Biochemical Hypoxia Down Regulates Activities and Expression of Super Oxide Dismutase and Catalase in Cerebral Cortex of Mice

Content Provider	Springer Nature Link
Author	Rani, Anupama Prasad, S.
Copyright Year	2014
Abstract	Hypoxia-induced oxidative stress is one of the major hallmark reasons underlying brain dysfunction. In the present manuscript, we have used CoCl$_{2}$-induced hypoxic mice to investigate alterations in the activities of chief antioxidative stress enzymes- superoxide dismutase (SOD) and catalase (CAT) and expression of their genes Sod1 and Cat in the cerebral cortex as this model has not been routinely used for carrying out such study. Hypoxia mimetic mice model was accordingly developed by oral CoCl$_{2}$ administration to mice and validated by analyzing alterations in the expression of the hypoxia inducible factor gene Hif-1α and its immediate responsive genes. Our Western blot data demonstrated that a dose of 40 mg/kg BW of CoCl$_{2}$ was able to generate hypoxia like condition in mice in which Hif-1α and its immediate responsive genes-glutamate transporter-1 (Slc2a1) and erythropoietin (Epo) expression were up regulated. Our in-gel assay data indicated that SOD and CAT activities significantly declined and it was associated with significant down regulation of Sod1 and Epo expression as evident from our semi quantitative RT-PCR and Western blot data, which might be correlated with up regulation of Hif-1α expression in the cerebral cortex of the CoCl$_{2}$-treated hypoxic mice. Our findings suggest that CoCl$_{2}$-induced hypoxic mouse model is useful for studying alterations in the anti oxidative enzymes and biochemical/molecular/neurobiological analysis of hypoxia-induced alterations in brain function.
Starting Page	1787
Ending Page	1796
Page Count	10
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	39
Issue Number	9
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2014-07-23
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cobalt chloride Hypoxia Brain Oxidative stress SOD Catalase Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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