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Three TRH-Like Molecules Are Released from Rat Hypothalamus In Vitro

Content Provider	Springer Nature Link
Author	Méndez, Milagros Cisneros, Miguel Baez, Armida Joseph Bravo, Patricia Charli, Jean Louis
Copyright Year	1999
Abstract	TRH-like immunoreactivity distinct from TRH is present in various tissues and fluids. In order to determine whether TRH-like molecules are secreted by the hypothalamus, we analyzed tissues and media from hypothalamic slices incubated in Krebs Ringer bicarbonate. Media from basal or high KCl conditions contained 3 TRH-like molecules evidenced by reverse phase high performance liquid chromatography followed by TRH radioimmunoassay. Peak I corresponded to authentic TRH (73% of total immunoreactivity) and peaks II and III had a higher retention time. These additional TRH-like forms were neither detected in hypothalamic tissue nor in tissue or medium from olfactory bulb. Gel filtration analysis of hypothalamic media revealed only one TRH-like peak eluting as TRH, suggesting that the molecular weights of peaks II and III are similar to that of TRH. Peak II retention time was similar to that of pglu-phe-proNH$_{2}$. We analysed if they could be produced by post secretory metabolism of TRH. Incubation of hypothalamic slices with [$^{3}$H-Pro]-TRH did not produce radioactive species comigrating with peaks II or III. However, it induced rapid degradation to [$^{3}$H-Pro]-his-prodiketopiperazine ([$^{3}$H]-HPDKP). Inhibitor profile suggested that pyroglutamyl aminopeptidase II, but not pyroglutamyl aminopeptidase I, is responsible for [$^{3}$H]-HPDKP production. These data are consistent with the hypothesis that pyroglutamyl aminopeptidase II is the main aminopeptidase degrading TRH in hypothalamic extracellular fluid. Furthermore, we suggest that the hypothalamus releases additional TRH-like molecules, one of them possibly pglu-phe-proNH$_{2}$, which may participate in control of adenohypophyseal secretions.
Starting Page	815
Ending Page	823
Page Count	9
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	24
Issue Number	7
e-ISSN	15736903
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neurosciences Neurology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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