NDLI: CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation

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CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation

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CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation

Content Provider	Springer Nature Link
Author	Long, Aaron Park, Ji H. Klimova, Nina Fowler, Carol Loane, David J. Kristian, Tibor
Copyright Year	2016
Abstract	Several enzymes in cellular bioenergetics metabolism require NAD$^{+}$ as an essential cofactor for their activity. NAD$^{+}$ depletion following ischemic insult can result in cell death and has been associated with over-activation of poly-ADP-ribose polymerase PARP1 as well as an increase in NAD$^{+}$ consuming enzyme CD38. CD38 is an NAD$^{+}$ glycohydrolase that plays an important role in inflammatory responses. To determine the contribution of CD38 activity to the mechanisms of post-ischemic brain damage we subjected CD38 knockout (CD38KO) mice and wild-type (WT) mice to transient forebrain ischemia. The CD38KO mice showed a significant amelioration in both histological and neurologic outcome following ischemic insult. Decrease of hippocampal NAD$^{+}$ levels detected during reperfusion in WT mice was only transient in CD38KO animals, suggesting that CD38 contributes to post-ischemic NAD$^{+}$ catabolism. Surprisingly, pre-ischemic poly-ADP-ribose (PAR) levels were dramatically higher in CD38KO animals compared to WT animals and exhibited reduction post-ischemia in contrast to the increased levels in WT animals. The high PAR levels in CD38 mice were due to reduced expression levels of poly-ADP-ribose glycohydrolase (PARG). Thus, the absence of CD38 activity can not only directly affect inflammatory response, but also result in unpredicted alterations in the expression levels of enzymes participating in NAD$^{+}$ metabolism. Although the CD38KO mice showed significant protection against ischemic brain injury, the changes in enzyme activity related to NAD$^{+}$ metabolism makes the determination of the role of CD38 in mechanisms of ischemic brain damage more complex.
Starting Page	283
Ending Page	293
Page Count	11
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	42
Issue Number	1
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2016-08-12
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nicotinamide dinucleotide Poly-ADP-ribose Ischemia Damage Brain Mouse Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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CD38 Knockout Mice Show Significant Protection Against Ischemic Brain Damage Despite High Level Poly-ADP-Ribosylation

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