NDLI: In Vitro Effect of H$_{2}$O$_{2}$, Some Transition Metals and Hydroxyl Radical Produced Via Fenton and Fenton-Like Reactions, on the Catalytic Activity of AChE and the Hydrolysis of ACh

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In Vitro Effect of H$_{2}$O$_{2}$, Some Transition Metals and Hydroxyl Radical Produced Via Fenton and Fenton-Like Reactions, on the Catalytic Activity of AChE and the Hydrolysis of ACh

Content Provider	Springer Nature Link
Author	Méndez Garrido, Armando Hernández Rodríguez, Maricarmen Zamora Ulloa, Rafael Correa Basurto, José Mendieta Wejebe, Jessica Elena Ramírez Rosales, Daniel Rosales Hernández, Martha Cecilia
Copyright Year	2014
Abstract	It is well known that the principal biomolecules involved in Alzheimer’s disease (AD) are acetylcholinesterase (AChE), acetylcholine (ACh) and the amyloid beta peptide of 42 amino acid residues (Aβ42). ACh plays an important role in human memory and learning, but it is susceptible to hydrolysis by AChE, while the aggregation of Aβ42 forms oligomers and fibrils, which form senile plaques in the brain. The Aβ42 oligomers are able to produce hydrogen peroxide (H$_{2}$O$_{2}$), which reacts with metals (Fe$^{2+}$, Cu$^{2+}$, Cr$^{3+}$, Zn$^{2+}$, and Cd$^{2+}$) present at high concentrations in the brain of AD patients, generating the hydroxyl radical ($^{·}$OH) via Fenton (FR) and Fenton-like (FLR) reactions. This mechanism generates high levels of free radicals and, hence, oxidative stress, which has been correlated with the generation and progression of AD. Therefore, we have studied in vitro how AChE catalytic activity and ACh levels are affected by the presence of metals (Fe$^{3+}$, Cu$^{2+}$, Cr$^{3+}$, Zn$^{2+}$, and Cd$^{2+}$), H$_{2}$O$_{2}$ (without Aβ42), and $^{ · }$OH radicals produced from FR and FLR. The results showed that the H$_{2}$O$_{2}$ and the metals do not modify the AChE catalytic activity, but the $^{·}$OH radical causes a decrease in it. On the other hand, metals, H$_{2}$O$_{2}$ and $^{·}$OH radicals, increase the ACh hydrolysis. This finding suggests that when H$_{2}$O$_{2}$, the metals and the $^{·}$OH radicals are present, both, the AChE catalytic activity and ACh levels diminish. Furthermore, in the future it may be interesting to study whether these effects are observed when H$_{2}$O$_{2}$ is produced directly from Aβ42.
Starting Page	2093
Ending Page	2104
Page Count	12
File Format	PDF
ISSN	03643190
Journal	Neurochemical Research
Volume Number	39
Issue Number	11
e-ISSN	15736903
Language	English
Publisher	Springer US
Publisher Date	2014-08-06
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alzheimer’s disease Acetylcholinesterase Acetylcholine Free radicals Metals Fenton and Fenton-like reactions Neurosciences Neurochemistry Biochemistry Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Cellular and Molecular Neuroscience

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