NDLI: Nanovesicle encapsulation of antimicrobial peptide P34: physicochemical characterization and mode of action on Listeria monocytogenes

Content Provider	Springer Nature Link
Author	da Silva Malheiros, Patrícia Sant’Anna, Voltaire Micheletto, Yasmine Miguel Serafini da Silveira, Nadya Pesce Brandelli, Adria
Copyright Year	2011
Abstract	Antimicrobial peptide P34, a substance showing antibacterial activity against pathogenic and food spoilage bacteria, was encapsulated in liposomes prepared from partially purified soybean phosphatidylcholine, and their physicochemical characteristics were evaluated. The antimicrobial activity was estimated by agar diffusion assay using Listeria monocytogenes ATCC 7644 as indicator strain. A concentration of 3,200 AU/mL of P34 was encapsulated in nanovesicles and stocked at 4 °C. No significant difference (p > 0.05) in the biological activity of free and encapsulated P34 was observed through 24 days. Size and PDI of liposomes, investigated by light scattering analysis, were on average 150 nm and 0.22 respectively. Zeta potential was −27.42 mV. There was no significant change (p > 0.05) in the physicochemical properties of liposomes during the time of evaluation. The liposomes presented closed spherical morphology as visualized by transmission electron microscopy (TEM). The mode of action of liposome-encapsulated P34 under L. monocytogenes cells was investigated by TEM. Liposomes appeared to adhere but not fuse with the bacterial cell wall, suggesting that the antimicrobial is released from nanovesicles to act against the microorganism. The effect of free and encapsulated P34 was tested against L. monocytogenes, showing that free bacteriocin inhibited the pathogen more quickly than the encapsulated P34. Liposomes prepared with low-cost lipid showed high encapsulation efficiency for a new antimicrobial peptide and were stable during storage. The mode of action against the pathogen L. monocytogenes was characterized.
Starting Page	3545
Ending Page	3552
Page Count	8
File Format	PDF
ISSN	13880764
Journal	Journal of Nanoparticle Research
Volume Number	13
Issue Number	8
e-ISSN	1572896X
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-02-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antimicrobial Bacteriocin Liposome Nanovesicles Morphology Mode of action Microbiology Inorganic Chemistry Optics, Optoelectronics, Plasmonics and Optical Devices Physical Chemistry Characterization and Evaluation of Materials Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Bioengineering Modeling and Simulation

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