NDLI: Molecular Analysis of Candida albicans Isolates from Clinical Specimens

Content Provider	Springer Nature Link
Author	Gurbuz, Melahat Kaleli, Ilknur
Copyright Year	2009
Abstract	The aim of this study was to genotype Candida albicans strains isolated from various clinical specimens by using CA-INT-R and CA-INT-L primer pairs designed to span the region that includes the site of the transposable group-1 intron in the 25S rRNA gene. A total of 194 C. albicans isolates (28 invasive and 166 noninvasive) were genotyped. The frequencies of genotypes A, B, C and D were found as 51.0, 29.4, 19.1 and 0.5%, respectively. Statistically significant difference was determined between frequency of genotype distribution between invasive and noninvasive isolates (P < 0.001). Genotype C was more prevalent among invasive isolates while genotype A was in noninvasive ones. Furthermore, six different subtypes were determined among genotype A C. albicans isolates by restriction endonuclease analysis using a previously constructed differentiation scheme consisting of HaeIII and MspI digestions. This study demonstrated the genetic diversity of clinical isolates of C. albicans in our hospital.
Starting Page	261
Ending Page	267
Page Count	7
File Format	PDF
ISSN	0301486X
Journal	Mycopathologia
Volume Number	169
Issue Number	4
e-ISSN	15730832
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-12-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Candida albicans Candida dubliniensis Genotyping 25S Invasiveness Plant Sciences Microbial Ecology Eukaryotic Microbiology Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Veterinary Microbiology Applied Microbiology and Biotechnology Agronomy and Crop Science

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