NDLI: Candida albicans Amphotericin B-Tolerant Persister Formation is Closely Related to Surface Adhesion

Content Provider	Springer Nature Link
Author	Sun, Jing Li, Zhigang Chu, Haoyue Guo, Jing Jiang, Guangshui Qi, Qingguo
Copyright Year	2015
Abstract	Candida albicans persisters have so far been observed only in biofilm environment; the biofilm element(s) that trigger(s) persister formation are still unknown. In this study, we tried to further elucidate the possible relationship between C. albicans persisters and the early phases of biofilm formation, especially the surface adhesion phase. Three C. albicans strains were surveyed for the formation of persisters. We tested C. albicans persister formation dynamically at different time points during the process of adhesion and biofilm formation. The number of persister cells was determined based on an assessment of cell viability after amphotericin B treatment and colony-forming unit assay. None of the planktonic cultures contained persisters. Immediately following adhesion of C. albicans cells to the surface, persister cells emerged and the proportion of persisters reached a peak of 0.2–0.69 % in approximately 2-h biofilm. As the biofilm matured, the proportion of persisters decreased and was only 0.01–0.02 % by 24 h, while the number of persisters remained stable with no significant change. Persisters were not detected in the absence of an attachment surface which was pre-coated. Persisters were also absent in biofilms that were scraped to disrupt surface adhesion prior to amphotericin B treatment. These results indicate that C. albicans antifungal-tolerant persisters are produced mainly in surface adhesion phase and surface adhesion is required for the emergence and maintenance of C. albicans persisters.
Starting Page	41
Ending Page	49
Page Count	9
File Format	PDF
ISSN	0301486X
Journal	Mycopathologia
Volume Number	181
Issue Number	1-2
e-ISSN	15730832
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-09-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Candida albicans Persisters Biofilm Adhesion Microbiology Medical Microbiology Eukaryotic Microbiology Microbial Ecology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Veterinary Microbiology Applied Microbiology and Biotechnology Agronomy and Crop Science

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