NDLI: An insight into the genes involved in secoiridoid biosynthesis in Gentiana macrophylla by RNA-seq

Content Provider	Springer Nature Link
Author	Hua, Wenping Zheng, Peng He, Yihan Cui, Langjun Kong, Weiwei Wang, Zhezhi
Copyright Year	2014
Abstract	The dried root of Gentiana macrophylla is a well-known traditional Chinese herbal medicine for treating jaundice, hepatitis, and stomachic and choleretic ailments. However, natural sources are now in short supply. A lack of information about its genetic background has been a great hindrance to producing its active constituents via genetic engineering. We performed RNA-seq to obtain 42,918 unigenes (average length = 667 bp) in its transcriptome. Of these, 32,141 (74.89 %) were annotated and 2,339 unigenes were assigned to secondary-metabolite pathways. In all, 114 putative unigenes involved in secoiridoid biosynthesis were identified in our transcriptome library. A Blast X search against the Arabidopsis gene regulatory information server showed that 4,413 unigenes are homologous to transcription factor genes from Arabidopsis. Organ-specific genes and candidate gene expression profiles were also investigated with digital gene expression technology. Quantitative PCR was used to verify the expression patterns of several novel transcripts involved in secoiridoid biosynthesis. Our results not only enrich the gene resource but will also benefit research into the molecular genetics and functional genomics of this species.
Starting Page	4817
Ending Page	4825
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	7
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-04-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gentiana macrophylla Transcriptome Secoiridoid biosynthesis DGE Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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