NDLI: Protein expression patterns in two Spiraea species in response to cold treatment

Content Provider	Springer Nature Link
Author	Liu, H. M. Fang, L. Che, Y. S. Wu, F. Z. Yang, C. P.
Copyright Year	2014
Abstract	We analyzed the different cold-resistance species Spiraea trichocarpa Nakai and Spiraea bumalda ‘Goldmound’ for low-temperature protein expression, protein types identification, and investigated the cold resistance mechanisms under different levels of low temperature by two-dimensional gel electrophoresis (2-DE) and mass spectrometry. An average of 668 and 559 protein spots were detected by 2-DE of S. bumalda ‘Goldmound’ and S. trichocarpa Nakai, respectively, under different low-temperature treatments. Matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy identified 48 proteins, with varying expression, related to metabolism, amino acid synthesis, transportation, stress responses and oxidation–reduction reactions. The results showed that the photosynthesis of S. bumalda ‘Goldmound’ had been affected, enzymes (RuBisCO large and small subunits) involved in the Calvin cycle were up- and down-regulated, and ATP synthase in photophosphorylation was down-regulated. Cytosolic malate dehydrogenase expression weakened in the TCA cycle, while amino acid synthesis strengthened. The activity of four antioxidant enzymes (superoxide dismutase [Cu–Zn], L-ascorbate peroxidase, glutathione peroxidase and peroxidase) was reduced under varying low temperatures. Enzymes (ribulose-bisphosphate carboxylase and RuBisCO small chain precursor) involved in the photosynthesis of S. trichocarpa Nakai showed obvious up- and down-regulation under low temperatures. Cold treatment influenced the photosynthesis of S. trichocarpa Nakai and S. bumalda ‘Goldmound’, but the results showed significant differences between the two species, which were supposed to the fact that low temperature modified the metabolic mechanisms and led to the weaker cold resistance in S. bumalda ‘Goldmound’ than in S. trichocarpa Nakai.
Starting Page	4533
Ending Page	4547
Page Count	15
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	41
Issue Number	7
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-03-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Spiraea L. Low temperature treatments 2-DE and MS analysis Protein identification Cold resistant mechanism Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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