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| Content Provider | Springer Nature Link |
|---|---|
| Author | Fang, Jingye Liu, Yong Zhang, Change Yang, Guangxiao Li, Kexiu Shewry, Peter R. He, Guangyuan |
| Copyright Year | 2008 |
| Abstract | A novel gene encoding an x-type high molecular weight glutenin subunit (HMW-GS), designated 1Dx1.1 $^{ t }$, was isolated from Aegilops tauschii. It is the largest HMW-GS gene reported so far in this species and its product has a slower mobility than that of subunit 1Ax1 in SDS-PAGE. The open reading frame (ORF) of the gene was 2,628 bp, encoding a protein of 874 amino acid residues. Comparisons of amino acid sequences showed that subunit 1Dx1.1$^{t}$ had high similarity with other 1Dx subunits but also had two unique characteristics. Firstly, a tripeptide of consensus LQE present in the N-terminal domains of other 1Dx subunits was absent from subunit Dx1.1$^{t}$. Secondly, three copies of tandem duplications of the tripeptide motif GQQ and a novel tripeptide sequence (GQL) were present in its central repetitive domain. Phylogenetic analysis showed that subunit 1Dx1.1$^{t}$ clustered with other known 1Dx subunits. |
| Starting Page | 1195 |
| Ending Page | 1202 |
| Page Count | 8 |
| File Format | |
| ISSN | 03014851 |
| Journal | Molecular Biology Reports |
| Volume Number | 36 |
| Issue Number | 5 |
| e-ISSN | 15734978 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2008-07-02 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aegilops tauschii HMW-GS Bacterial expression Evolutionary analysis Wheat quality improvement Animal Biochemistry Animal Anatomy / Morphology / Histology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Molecular Biology |
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