NDLI: Structural characterization of the involvement of SigC in the regulation of the gene expression of pathogenic Mycobacterium ulcerans

Content Provider	Springer Nature Link
Author	Bagchi, Angshuman
Copyright Year	2008
Abstract	Mycobacterium ulcerans is the causative agent of the Buruli ulcers in humans. This disease is a devastating necrotic disease of the subcutaneous tissue. Currently there are very few drugs to prevent the Buruli ulcers. This human pathogen is carried by aquatic insects. The gene sequence of this pathogenic organism has recently been identified. In the present study an attempt has been made to analyze the structural characteristics of the SigC protein from Mycobacterium ulcerans. Sequence analyses of SigC reveal that it belongs to the family of sigma factors, which are involved in DNA binding at promoter sites, and play a role in transcription initiation. Homology modeling was employed to construct the three-dimensional structure of the protein and the model was analyzed. SigC protein has a distinct helix-turn-helix DNA binding motif. It is compared to other members of the DNA binding proteins. The modeled structure of SigC has been used to dock on the promoter DNA to predict the favourable binding interactions of the protein, which are responsible for the gene expressions in the organism. These interactions have been used to predict the plausible mechanism of the process. Since this is the first structural work regarding the interactions of SigC protein and its promoter DNA, this study may contribute in the realm of structure based drug design approaches to produce drugs against this harmful organism to control its proliferation and spreading of Buruli ulcer.
Starting Page	965
Ending Page	969
Page Count	5
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	36
Issue Number	5
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-03
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Molecular modeling SigC protein Molecular docking Pathogenic organism Drug design Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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