NDLI: Cloning of sarco/endoplasmic reticulum Ca$^{2+}$-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress

Content Provider	Springer Nature Link
Author	Wang, Yanhong Luo, Peng Zhang, Lvping Hu, Chaoqu Ren, Chunhua Xia, Jianjun
Copyright Year	2013
Abstract	Sarco/endoplasmic reticulum Ca$^{2+}$-ATPase (SERCA) is an intracellular membrane bound enzyme that utilizes the free energy of ATP to transport Ca$^{2+}$ against a concentration gradient. In the present study, a new SERCA gene (LvSERCA) from white shrimp (Litopenaeus vannamei) was cloned using suppression subtractive hybridization and rapid amplification of cDNA ends. The full-length cDNA of LvSERCA contained an open reading frame of 3,009 bp coding for 1,002 amino acids with a calculated molecular weight of approximately 109.8 kDa. The identity analysis of the amino acid sequence of LvSERCA showed that it is highly conserved with 10 transmembrane α-helices, one P-domain, one A-domain and one N-domain. The phylogenetic analysis revealed that LvSERCA is similar to other Arthropoda SERCA proteins. The mRNA levels of LvSERCA under salinity stress (3 and 40 g L$^{−1}$) were analyzed by reverse transcription PCR and quantitative real-time PCR. The results showed that LvSERCA was expressed in all tissues detected. LvSERCA mRNA levels were significantly higher under hyper-salinity than hypo-salinity. These results highlight that Ga$^{2+}$-ATPase plays an essential role in adjustment salinity stress, which may be useful for selective breeding of L. vannamei.
Starting Page	6213
Ending Page	6221
Page Count	9
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	40
Issue Number	11
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cloning Litopenaeus vannamei Quantitative real-time RT-PCR Rapid amplification of cDNA ends Sarco/endoplasmic reticulum Ca$^{2+}$-ATPase Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology

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