NDLI: Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Content Provider	Springer Nature Link
Author	Peng, Ting Sun, Xiaochun Mumm, Rita H.
Copyright Year	2013
Abstract	From a breeding standpoint, multiple trait integration (MTI) is a four-step process of converting an elite variety/hybrid for value-added traits (e.g. transgenic events) using backcross breeding, ultimately regaining the performance attributes of the target hybrid along with reliable expression of the value-added traits. In the light of the overarching goal of recovering equivalent performance in the finished conversion, this study focuses on the first step of MTI, single event introgression, exploring the feasibility of marker-aided backcross conversion of a target maize hybrid for 15 transgenic events, incorporating eight events into the female hybrid parent and seven into the male parent. Single event introgression is conducted in parallel streams to convert the recurrent parent (RP) for individual events, with the primary objective of minimizing residual non-recurrent parent (NRP) germplasm, especially in the chromosomal proximity to the event (i.e. linkage drag). In keeping with a defined lower limit of 96.66 % overall RP germplasm recovery (i.e. ≤120 cM NRP germplasm given a genome size of 1,788 cM), a breeding goal for each of the 15 single event conversions was developed: <8 cM of residual NRP germplasm across the genome with ~1 cM in the 20 cM region flanking the event. Using computer simulation, we aimed to identify optimal breeding strategies for single event introgression to achieve this breeding goal, measuring efficiency in terms of number of backcross generations required, marker data points needed, and total population size across generations. Various selection schemes classified as three-stage, modified two-stage, and combined selection conducted from BC1 through BC3, BC4, or BC5 were compared. The breeding goal was achieved with a selection scheme involving five generations of marker-aided backcrossing, with BC1 through BC3 selected for the event of interest and minimal linkage drag at population size of 600, and BC4 and BC5 selected for the event of interest and recovery of the RP germplasm across the genome at population size of 400, with selection intensity of 0.01 for all generations. In addition, strategies for choice of donor parent to facilitate conversion efficiency and quality were evaluated. Two essential criteria for choosing an optimal donor parent for a given RP were established: introgression history showing reduction of linkage drag to ~1 cM in the 20 cM region flanking the event and genetic similarity between the RP and potential donor parents. Computer simulation demonstrated that single event conversions with <8 cM residual NRP germplasm can be accomplished by BC5 with no genetic similarity, by BC4 with 30 % genetic similarity, and by BC3 with 86 % genetic similarity using previously converted RPs as event donors. This study indicates that MTI to produce a ‘quality’ 15-event-stacked hybrid conversion is achievable. Furthermore, it lays the groundwork for a comprehensive approach to MTI by outlining a pathway to produce appropriate starting materials with which to proceed with event pyramiding and trait fixation before version testing.
Starting Page	89
Ending Page	104
Page Count	16
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	33
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-08-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Computer simulation Multiple trait integration Marker-aided backcross Single event introgression Breeding strategy Linkage drag Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Optimized breeding strategies for multiple trait integration: III. Parameters for success in version testing

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Molecular marker analysis of the linkage drag around the FAE1 loci of Brassica juncea during conventional backcross breeding

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Optimized breeding strategies for multiple trait integration: III. Parameters for success in version testing

Enhancing spot blotch resistance in wheat by marker-aided backcross breeding

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Similar Documents

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Optimized breeding strategies for multiple trait integration: III. Parameters for success in version testing

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Molecular marker analysis of the linkage drag around the FAE1 loci of Brassica juncea during conventional backcross breeding

Optimized breeding strategies for multiple trait integration: II. Process efficiency in event pyramiding and trait fixation

Optimized breeding strategies for multiple trait integration: III. Parameters for success in version testing

Enhancing spot blotch resistance in wheat by marker-aided backcross breeding

Optimized breeding strategies for multiple trait integration: I. Minimizing linkage drag in single event introgression