NDLI: Over-expression of the Gr5 $_{ aroA }$ gene from glyphosate-contaminated soil confers high tolerance to glyphosate in tobacco

Content Provider	Springer Nature Link
Author	Wang, Jin Zuo, Kaijing Lu, Wei Zhu, Yu Ye, Chun Lin, Min Tang, Kexuan
Copyright Year	2013
Abstract	Herbicide resistance is the most widely used transgenic crop trait for broad-spectrum control of weeds. Here we report a novel 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (Gr5 $_{ aroA }$) isolated from glyphosate-contaminated soil. The full Gr5 $_{ aroA }$ gene was 1,819 bp and contained a 1,341-bp open reading frame encoding a 47-kDa protein. Phylogenetic analysis showed that Gr5$_{aroA}$ is a class I EPSPS even though most such enzymes are naturally sensitive to glyphosate. Interestingly, Gr5$_{aroA}$ protein contained highly conserved PEP and S3P binding residues (Glu-351) and several motifs insensitive to glyphosate. Transgenic Gr5 $_{ aroA }$ plants (T $_{0}$) grew normally and produced seeds which we treated with a high-glyphosate solution (4× recommended spray). Analysis of the T $_{1}$ progenies showed that Gr5 $_{ aroA }$ was inherited at a Mendelian 3:1 segregation ratios and that glyphosate tolerance in T $_{1}$ plants was unchanged. Our results show the Gr5 $_{ aroA }$ gene to be a promising candidate for the development of commercial transgenic crops with high glyphosate tolerance.
Starting Page	197
Ending Page	208
Page Count	12
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	33
Issue Number	1
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-10-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gr5$_{aroA}$ Glyphosate tolerance Transformation Transgenic plant Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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