NDLI: Molecular tagging and mapping of the erect panicle gene in rice

Content Provider	Springer Nature Link
Author	Kong, Fan Na Wang, Jia Yu Zou, Ji Cheng Shi, Li Xue Jin, Min Xu, Zheng Jin Wang, Bin
Copyright Year	2006
Abstract	Erect panicle (EP) is one of the more important traits of the proposed ideotype of high-yielding rice. Several rice cultivars with the EP phenotype, which has been reported to be controlled by a dominant gene, have been successfully developed and released for commercial production in North China. To analyze the inheritance of the EP trait, we generated segregating F$_{2}$ and BC$_{1}$F$_{1}$ populations by crossing an EP-type variety, Liaojing 5, and a curved-panicle-type variety, Fengjin. Our results confirmed that a dominant gene controls the EP trait. Simple-sequence repeat (SSR) and bulked segregant analyses of the F$_{2}$ population revealed that the EP gene is located on chromosome 9, between two newly developed SSR markers, RM5833-11 and RM5686-23, at a genetic distance of 1.5 and 0.9 cM, respectively. Markers closer to the EP gene were developed by amplified fragment length polymorphism (AFLP) analysis with 128 AFLP primer combinations. Three AFLP markers were found to be linked to the EP gene, and the nearest marker, E-TA/M-CTC$_{200}$, was mapped to the same location as SSR marker RM5686-23, 1.5 cM from the EP gene. A local map around the EP gene comprising nine SSR and one AFLP marker was constructed. These markers will be useful for marker-assisted selection (MAS) for the EP trait in rice breeding programs.
Starting Page	297
Ending Page	304
Page Count	8
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	19
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-12-06
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	AFLP EP gene Molecular markers SSR Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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