NDLI: Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza Sativa L.

Content Provider	Springer Nature Link
Author	Qin, Qiu lin Liu, Jin ge Zhang, Zhen Peng, Ri he Xiong, Ai sheng Yao, Quan hong Chen, Jian min
Copyright Year	2006
Abstract	A previous study had indicated that the transcription factors DREB/CBF (DRE-binding protein/C-repeat binding factor) play important roles in the expression of many stress inducible genes under cold-temperature, dehydration and high-salt conditions. In this study, we have isolated a cDNA clone that encoded a DRE-binding protein from rice cDNA library using the yeast one-hybrid system with DRE cis-acting element in the promoter region of rd29A gene as bait. Sequence analysis of the deduced amino acid sequence showed this protein was a putative AP2/EREBP transcription factor with a conserved AP2/EREBP domain and a potential nuclear localization signal (NLS). Expression pattern studies of this DRE-binding protein revealed that this gene was not only strongly induced by cold-temperature as reported by previous study but also induced by high-temperature as well. For the purpose of analyzing this gene conveniently, we attempted to apply the codon optimization method to reconstruct the gene of transcription factor in plants. A new sequence having decreasing GC contents, secondary structures, optimized codons and identical amino acid sequence with native gene was synthesized, which named OsDREB1BI, and then this optimized gene was transformed into Arabidopsis thaliana cv. Columbia by floral dip method. Results indicated that the OsDREB1BI gene was over-expressed in transgenic plants under cold and high-temperature, meanwhile, those transgenic plants also revealed freezing and heat tolerance. These might lay a strong foundation for exploiting the freezing and heat tolerance of rice and other species.
Starting Page	329
Ending Page	340
Page Count	12
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	19
Issue Number	4
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2006-11-21
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DRE-binding protein Rice Codon optimization Abiotic stress tolerance Transgenic plant Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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