NDLI: Evaluation of genes to reduce seed size in shape Arabidopsis and tobacco and their application to shape Citrus

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Evaluation of genes to reduce seed size in shape Arabidopsis and tobacco and their application to shape Citrus

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Molecular Breeding : Volume 4, Issue 2, April 1998

Molecular Breeding : Volume 4, Issue 1, February 1998

Molecular Breeding : Volume 3

Evaluation of genes to reduce seed size in shape Arabidopsis and tobacco and their application to shape Citrus

Content Provider	Springer Nature Link
Author	Koltuw, Anna M. Brennan, Peter Bond, James E. Barker, Susan J.
Copyright Year	1998
Abstract	Seedlessness is a highly desirable characteristic in fresh fruit. Marketability of a fruit as seedless does not require complete absence of seeds as long as the seed structures are imperceptible during consumption. Chimaeric genes comprised of soybean β-conglycinin seed storage protein gene promoters linked to the bacterial RNase gene, Barnase, were tested for their efficacy to cause seed death and decrease seed size in tobacco and Arabidopsis. These species were used because they undergo two distinct seed developmental pathways and produce albuminous and exalbuminous seeds, respectively. In both species, the death of embryo and endosperm tissues occurred, resulting in a dominant seed lethal phenotype with segregation distortion. Reduction in seed size was only observed in Arabidopsis seeds and the phenotype resembled that of stenospermocarpic seeds in grape. Some transformants of both species were male-sterile and this correlated with the expression of the gene in anthers indicating that expression of the gene is not strictly seed-specific. The promoters also direct expression of a linked GUS gene to Citrus embryos of various developmental stages, and Citrus forms exalbuminous seeds, therefore, the Barnase constructions may be useful in eliciting a reduction in seed size of around 75% of the seeds found in the fruit. This may be sufficient to warrant marketing as ‘less seedy’ if trials in the cultivar of interest indicate that the smaller seeds are less detectable to the consumer. Abbreviations: GUS, β-glucuronidase; PCR, polymerase chain reaction; DMSO, dimethyl sulfoxide; DTA, diphtheria toxin-A chain; CFDA, 5(6)-carboxy-fluorescein di-acetate; CG, β-conglycinin; DAP, days after pollination; FAA, formaldehyde-acetic acid alcohol fixative.
Starting Page	235
Ending Page	251
Page Count	17
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	4
Issue Number	3
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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