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DFLP, SSCP, and SSR markers for Δ9-stearoyl-acyl carrier protein desaturases strongly expressed in developing seeds of sunflower: intron lengths are polymorphic among elite inbred lines

The extent of parental genotypic divergence determines maximal heterosis by increasing fertility in inter-subspecific hybrids of rice (Oryza sativa L.)

Analyses of quantitative trait loci associated with resistance to shape Sclerotinia sclerotiorum in sunflowers (shape Helianthus annuus L.) using molecular markers

Marker-assisted selection for two rust resistance genes in sunflower

Evaluation of genes to reduce seed size in shape Arabidopsis and tobacco and their application to shape Citrus

Paternal transmission of a seed size reduction gene varies with age of a primary transformant and seed set is also influenced by gene expression in maternal tissues

SSR heterogenic patterns of parents for marking and predicting heterosis in rice breeding

Introduction of the cDNA for shape Arabidopsis glycerol-3-phosphate acyltransferase (GPAT) confers unsaturation of fatty acids and chilling tolerance of photosynthesis on rice

Molecular Breeding : Volume 4, Issue 2, April 1998

Molecular Breeding : Volume 4, Issue 1, February 1998

Molecular Breeding : Volume 3

DFLP, SSCP, and SSR markers for Δ9-stearoyl-acyl carrier protein desaturases strongly expressed in developing seeds of sunflower: intron lengths are polymorphic among elite inbred lines

Content Provider	Springer Nature Link
Author	Hongtrakul, Vipa Slabaugh, Mary B. Knapp, Steven J.
Copyright Year	1998
Abstract	Stearoyl-acyl carrier protein desaturase (SAD, EC 1.14.99.6) produces oleic acid (18:1 Δ9) by desaturating 18:0. SAD genes have been targets for breeding and engineering oilseed crops with increased stearic acid (18:0). Our aim was to clone, describe, and develop genetic markers for the SAD genes of sunflower (Helianthus annuus L.). Nineteen SAD cDNA clones were partially sequenced and found to belong to two groups. Full-length cDNAs from each group (SAD6 and SAD17) were completely sequenced. The amino acid identity of SAD6 and SAD17 was 89%. Both genes were strongly expressed in developing seeds, moderately expressed in leaves and flowers, and weakly expressed in cotyledons, roots, and stems. One intron was found in SAD6 and two introns were found in SAD17. The SAD introns from two inbred lines (HA370 and HA372) were sequenced and found to vary in length and nucleotide sequence. The length variants were caused by monomeric repeat length differences, insertions, and deletions. Three long poly-T repeats (T$_{9}$ to T$_{39}$) were found in one of the SAD17 introns. Three short adjacent CA repeats were found in the 5′-untranslated region of SAD6. DNA fragment length polymorphism (DFLP), single-strand conformational polymorphism (SSCP), and simple sequence repeat (SSR) markers were developed for SAD6 and SAD17 by developing primers to flank introns or the CA repeats. Two of six DFLP, four of six SSCP, and one of two SSR markers were polymorphic among eight elite inbred lines. The polymorphic information contents for DFLP, SSCP, and SSR markers were 0.18, 0.37, and 0.30, respectively. Most of the polymorphisms were caused by intron fragment length polymorphisms. Introns may be an excellent source of hypervariable markers in sunflower and other crop plants.
Starting Page	195
Ending Page	203
Page Count	9
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	4
Issue Number	3
e-ISSN	15729788
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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