NDLI: Characterization of a new allelic mutant of DWARF3 in rice and analysing its function and stability in the presence of strigolactone

Content Provider	Springer Nature Link
Author	Liang, Yueyang Wang, Shiquan Huang, Xiaoxi Wang, Haipeng Liu, Fenlong Li, Shuangcheng Zhu, Jun Deng, Qiming Liu, Huainian Zheng, Aiping Wang, Lingxia Li, Ping
Copyright Year	2017
Abstract	Strigolactones (SLs) are important intrinsic growth regulators that control plant architecture by coordinating shoot and root development. Recent studies demonstrate that SL signals act via targeting the degradation protein DWARF53 (D53) family of chaperonin-like proteins. This process requires DWARF14 (D14) as strigolactones signal receptor and DWARF3 (D3) forming Skp-Cullin-F-box (SCF) complex as ubiquitin E3 ligase. Although the interactions of these signal components can be expected, where and how the SLs signalling occur within cells in a tissue-specific manner is still uncertain. In this study, we characterize a rice high-tillering dwarf mutant, ext.-M1B, displaying resistance to synthetic strigolactone mixture rac-GR24. Through genetic analysis, we find that ext.-M1B is a new allelic mutant of D3 with a nucleotide mutation resulting in a truncated protein of wide-type D3. We demonstrate that the mutation affects neither gene expression level nor the protein sub-cellular localization, whereas it disrupts the perception of SLs signal in ext.-M1B mutant. Moreover, we find that overexpression of D3 in wild type background causes no significant phenotype, but suppression of D3 by RNA interfering results in a clear phenocopy of SL mutants. By expressing fluorescent D3 fusion protein in rice, we first show that D3 is stable consistently in the nucleus with or without strigolactone treatment. Taken together, our data indicates that D3 encoding an F-box protein in nucleus, as a stable signal component response to strigolactone regulating rice shoot architecture.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	37
Issue Number	3
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-03-11
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	D3 ext.-M1B Rice Shoot architecture Strigolactone Plant Sciences Plant Genetics & Genomics Plant Pathology Plant Physiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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