NDLI: Superoxide dismutase: an all-purpose gene for agri-biotechnology

Content Provider	Springer Nature Link
Author	Gusta, Lawrence V. Benning, Nicole T. Wu, Guohai Luo, Ximing Liu, Xunjia Gusta, Michael L. McHughen, Alan
Copyright Year	2009
Abstract	The objective of this study was to evaluate the performance of transgenic canola (Brassica napus) plants over-expressing a wheat mitochondrial Mn superoxide dismutase (Mn SOD3.1) subjected to environmental stresses in the field and in controlled environments. Mn SOD3.1 was regulated by either CaMV 35S or Arabidopsis COR78 promoters. RT-PCR and a SOD enzyme activity assays demonstrated Mn SOD3.1 was expressed at both the mRNA and protein levels. Enzyme activity assays exhibited total SOD activity was up to 41.8% and up to 26.7% higher in the 35S:SOD3.1 and in the COR78:SOD3.1 transgenic plants than in the control, respectively. Germination studies, conducted at suboptimal (8°C) and optimal (23°C) temperatures, identified transgenic lines that germinated earlier than the control. In the field under drought conditions, several transgenic lines emerged earlier than the control. In both greenhouse and field environments, several transgenic lines were significantly taller than the control and over 50% of the transgenic canola lines flowered 7–14 days earlier than the control. Over expression of Mn SOD3.1 enhanced heat, drought and cold tolerance both in the field and under artificial stress conditions. This is one of the first tests conducted on transgenic canola plants subjected to field conditions.
Starting Page	103
Ending Page	115
Page Count	13
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	24
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-31
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Superoxide dismutase Abiotic stress Seedling vigor Genetic engineering Biotechnology Plant Physiology Plant Sciences Plant Pathology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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