NDLI: Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties

Content Provider	Springer Nature Link
Author	Sakthivel, K. Shobha Rani, N. Pandey, Manish K. Sivaranjani, A. K. P. Neeraja, C. N. Balachandran, S. M. Sheshu Madhav, M. Viraktamath, B. C. Prasad, G. S. V. Sundaram, R. M.
Copyright Year	2009
Abstract	Fragrance development in rice has been reported due to a 8-bp deletion in the exon 7 of badh2 gene located on Chromosome 8S. Multiplex markers targeting the functional InDel polymorphism was earlier reported for genotyping fragrance trait, but the marker was observed to be inconsistent and difficult to use. We have developed a simple, co-dominant, functional marker for fragrance trait, which can be resolved in an agarose gel and validated in Basmati and non-Basmati aromatic rice varieties and in a mapping population segregated for fragrance trait. The marker targets the InDel polymorphism in badh2 gene and amplifies 95 and 103 bp fragments in fragrant and non-fragrant genotypes, respectively. The newly developed marker was highly efficient in discriminating all fragrant and non-fragrant genotypes and showed perfect co-segregation with the trait of fragrance in the mapping population. We recommend the use of this simple, low-cost marker in routine genotyping for fragrance trait in large scale breeding materials and germplasm.
Starting Page	185
Ending Page	190
Page Count	6
File Format	PDF
ISSN	13803743
Journal	Molecular Breeding
Volume Number	24
Issue Number	2
e-ISSN	15729788
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-05-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oryza sativa Rice Fragrance Aroma badh2 Functional marker Biotechnology Plant Physiology Plant Sciences Plant Pathology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Plant Science Molecular Biology Biotechnology Agronomy and Crop Science

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