NDLI: Vitamin D$_{3}$ prevents the increase in ectonucleotidase activities and ameliorates lipid profile in type 1 diabetic rats

Content Provider	Springer Nature Link
Author	Calgaroto, Nicéia Spanholi Costa, Pauline Cardoso, Andréia Machado Pereira, Luciane Belmonte Vieira, Julia Marchi Dalegare, Diéssica Pelinson, Luana Paula Baldissarelli, Jucimara Morsch, Vera Maria Schetinger, Maria Rosa Chitolina
Copyright Year	2015
Abstract	This study was designed to assess the potential effect of vitamin D$_{3}$ (VD$_{3}$) in avoiding atherothrombosis by modulation of lipid metabolism and platelet activation in type 1 diabetic rats. Male wistar rats were divided into eight groups (n = 5–10): Control/Saline (Sal); Control/Metformin 500 mg/kg (Metf); Control/Vitamin D$_{3}$ 90 µg/kg (VD$_{3}$); Control/Metformin 500 mg/kg + VD$_{3}$ 90 µg/kg (Metf + VD$_{3}$); Diabetic/Saline (Sal); Diabetic/Metformin 500 mg/kg (Metf); Diabetic/Vitamin D$_{3}$ 90 µg/kg (VD$_{3}$); Diabetic/Metformin 500 mg/kg + VD$_{3}$ 90 µg/kg (Metf + VD$_{3}$). Treatments were administered during 30 days after diabetes induction with streptozotocin (STZ). After 31 days, the rats were euthanized and blood was collected and separated into serum and platelets, both used for lipid profile and ectonucleotidase activity assays, respectively. Ectonucleoside triphosphate phosphohydrolase (E-NTPDase), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), and 5′-nucleotidase and adenosine deaminase (E-ADA) were significantly higher in the Diabetic than in Control group. Treatment with Metf and/or VD$_{3}$ prevented the increase in NTPDase and E-NPP activities in diabetic rats. Only Metf + VD$_{3}$ significantly prevented the increase in 5′-nucleotidase. VD$_{3}$ alone, but not Metf, prevented the increase in ADA activity when compared to saline-treated diabetic rats. Treatment of rats with VD$_{3}$, Metf, and Metf + VD$_{3}$ was also effective in the prevention of lipid metabolism disorder in diabetic and was able to ameliorate lipid metabolism in non-diabetic rats. These results provide evidence for the potential of Metf and VD$_{3}$ in the treatment of platelet dysfunction and lipid metabolism impairment in T1D, which may be important in the control and prevention of atherothrombosis in diabetes.
Starting Page	11
Ending Page	21
Page Count	11
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	405
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Springer US
Publisher Date	2015-04-17
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cholecalciferol Type 1 diabetes Metformin Ectonucleotidases Atherothrombosis Biochemistry Medical Biochemistry Oncology Cardiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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