NDLI: Prohibitin suppresses renal interstitial fibroblasts proliferation and phenotypic change induced by transforming growth factor-β1

Content Provider	Springer Nature Link
Author	Guo, Wei Xu, Hong Chen, Jing Yang, Yong Jin, Jia Wei Fu, Rui Liu, Hai Mei Zha, Xi Liang Zhang, Zhi Gang Huang, Wen Yan
Copyright Year	2006
Abstract	Prohibitin (PHB), a potential tumor suppressor, has been shown to inhibit cell proliferation by repressing E2F-mediated transcription. But little is known about the role of PHB involved in tubulointerstitial fibrosis (TIF). Here, for the first time, we found PHB protein was positively expressed at normal renal tissues, strongly down-regulated in renal biopsy specimens, and negatively correlated with the expression of alpha-smooth-muscle actin (α-SMA) and with the degrees of tubulointerstitial lesions. Transforming growth factor-β1 (TGF-β1) is the most important profibrotic cytokine in the process of TIF and capable of inducing cell phenotypic change of interstitial fibroblasts characterized by the de novo expression of α-SMA. Confocal microscopy showed majority of PHB is located at cytoplasm as well as at nucleus in rat kidney fibroblasts cell (NRK-49F). As we found that PHB protein and mRNA expression were down-regulated in NRK-49F cells following TGF-β1 stimulation. We used transient transfection to over-express PHB protein and found that cells with increased PHB levels had a significant reduction in the percentage entering cell cycle and abolished de novo expression of α-SMA following TGF-β1 stimulation. Therefore, over-expression of PHB suppresses renal interstitial fibroblasts proliferation and cell phenotypic change induced by TGF-β1, which indicates PHB as a potential therapeutic target to halt the progression of TIF.
Starting Page	167
Ending Page	177
Page Count	11
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	295
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-10-17
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	prohibitin fibroblast transforming growth factor-β1 cell cycle cell phenotypic change tubulointerstitial lesions Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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