NDLI: Dual mechanism of autoregulation of protein kinase C in myocardial ischemia

Content Provider	Springer Nature Link
Author	Simonis, Gregor Schoen, Steffen P. Braun, Martin U. Lichte, Stefan Marquetant, Rainer Strasser, Ruth H.
Copyright Year	2006
Abstract	Background: Recently, a dual activation mechanism of protein kinase C (PKC) in ischemia has been reported, consisting of early translocation and late expressional regulation. Moreover, autophosphorylation of the enzyme has been shown in vitro during its activation. This study aimed to show modes of late activation of PKC in myocardial ischemia in intact hearts. Methods and Results: Isolated perfused hearts of male Wistar rats were used. A: To examine if the early translocation of PKC influences the late transcriptional activation, hearts were treated with the PKC-inhibitor Bisindolylmaleimid (BIS, 0.25 μM) before the onset of ischemia and then subjected to ischemia (30 min). PKC-isoform mRNA was quantified by RT-PCR. In these experiments, ischemia leads to a selective increase of mRNA specific for the isoforms PKC-δ and PKC-ε (163% and 168% of control, p < 0.05). This ischemia-induced upregulation could be completely blocked by BIS given before the onset of ischemia. B: To test the capacity of PKC to undergo phosphorylation during ischemia, hearts were perfused with [$^{32}$P]-phosphorus and then subjected to ischemia. Ischemia (30 min) induced a significant 3-fold increase of PKC phosphorylation. Stimulation of heart with the PKC-activator tetradecanoylphorbol-13-acetate (TPA) lead to a comparable phosphorylation, suggesting that ischemia leads to autophosphorylation of PKC. Conclusion: Ischemia activates two distinct forms of autoregulation of PKC. The expressional upregulation of PKC-δ and PKC-ε is dependent on early activation of the enzyme. At the same time, processes of enzyme phosphorylation occur. Both the mechanisms may contribute to enzyme activation processes beyond the classical enzyme translocation.
Starting Page	121
Ending Page	128
Page Count	8
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	295
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers-Plenum Publishers
Publisher Date	2006-08-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	protein kinase C ischemia phosphorylation expressional regulation Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differential regulation of protein kinase C isoforms of macrophages by pathogenic and non-pathogenic mycobacteria

Protein kinase C expression and subcellular distribution in chronic myocardial ischemia: Comparison of two different canine models

Regulation of protein kinase C isozymes in volume overload cardiac hypertrophy

Down-regulation of neuropathy target esterase by protein kinase C activation with PMA stimulation

Regulation of tyrosine kinase activity during capacitation in goat sperm

Protein kinase network in the regulation of phosphorylation and dephosphorylation of smooth muscle myosin light chain

Up-regulation and redistribution of protein kinase C-δ in chronically hypoxic heart

Molecular biology of protein kinase C signaling in cardiac myocytes

Protein-, gene-, and cell-based therapeutic angiogenesis for the treatment of myocardial ischemia

Dual mechanism of autoregulation of protein kinase C in myocardial ischemia

Similar Documents

Differential regulation of protein kinase C isoforms of macrophages by pathogenic and non-pathogenic mycobacteria

Protein kinase C expression and subcellular distribution in chronic myocardial ischemia: Comparison of two different canine models

Regulation of protein kinase C isozymes in volume overload cardiac hypertrophy

Down-regulation of neuropathy target esterase by protein kinase C activation with PMA stimulation

Regulation of tyrosine kinase activity during capacitation in goat sperm

Protein kinase network in the regulation of phosphorylation and dephosphorylation of smooth muscle myosin light chain

Up-regulation and redistribution of protein kinase C-δ in chronically hypoxic heart

Molecular biology of protein kinase C signaling in cardiac myocytes

Protein-, gene-, and cell-based therapeutic angiogenesis for the treatment of myocardial ischemia

Dual mechanism of autoregulation of protein kinase C in myocardial ischemia