NDLI: Expression of GABA$_{A}$ and GABA$_{B}$ receptors in rat growth plate chondrocytes: Activation of the GABA receptors promotes proliferation of mouse chondrogenic ATDC5 cells

Content Provider	Springer Nature Link
Author	Tamayama, Takumi Maemura, Kentaro Kanbara, Kiyoto Hayasaki, Hana Yabumoto, Yasuaki Yuasa, Masayoshi Watanabe, Masahito
Copyright Year	2005
Abstract	Our previous study showed the local production of γ-aminobutyrate (GABA) in hypertrophic-zone chondrocytes of the rat tibial growth plate, an important long bone growth site. The aim of this study was to identify the presence of GABA receptors in growth plate chondrocytes by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Chondrocytes expressed both GABA$_{A}$ and GABA$_{B}$ receptor subunit mRNAs as well as the corresponding proteins necessary for the assembly of functional receptors. The GABA$_{A}$ receptor subunits detected included α1–α4, α6, β1–β3, and δ, and both R1 and R2 subunits of GABA$_{B}$ receptors were detected. All receptor subunits were expressed in chondrocytes of the proliferative and hypertrophic zones. These results suggest that GABA is an autocrine/paracrine factor that regulates the physiological state of the growth plate. Subsequent studies with the mouse chondrogenic cell line ATDC5 showed the presence of mRNAs and the corresponding proteins for GABA$_{A}$ receptor α1, β2, and β3 subunits and GABA$_{B}$ receptor R1 and R2 subunits. GABA, muscimol (a GABA$_{A}$ receptor agonist), and baclofen (a GABA$_{B}$ receptor agonist) increased 5-bromodeoxyuridine (BrdU) incorporation into ATDC5 cells. The effect of muscimol was blocked by bicuculline (a GABA$_{A}$ receptor antagonist), and the effect of baclofen was blocked by CGP 35348 (a GABA$_{B}$ receptor antagonist). These results suggest that GABA contributes to the ATDC5 cell proliferation via GABA$_{A}$ and GABA$_{B}$ receptors and these mechanisms may be involved in cartilaginous cell growth.
Starting Page	117
Ending Page	126
Page Count	10
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	273
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2005-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	growth plate chondrocyte GABA GABA$_{A}$ receptor GABA$_{B}$ receptor proliferation Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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