NDLI: Arrest of transcription following anoxic exposure in a marine mollusc

Content Provider	Springer Nature Link
Author	Larade, Kevin Storey, Kenneth B.
Copyright Year	2007
Abstract	The intertidal marine snail, Littorina littorea, is an anoxia tolerant species that endures long-term oxygen deprivation using a suite of compensatory metabolic adaptations that includes overall metabolic rate depression. Nuclear run-off assays were used to quantify the relative rates of mRNA transcription in nuclear extracts from hepatopancreas of aerobic and anoxic snails. Total [$^{32}$P]-UTP incorporation into RNA by nuclei from 48 h anoxic snails ranged from 42 to 50% of that observed for nuclei from normoxic snails. When this data is transformed with respect to incubation time, the rate of [$^{32}$P]-UTP incorporation by nuclei from 48 h anoxic snails showed a decrease of 68% as compared with the normoxic level. Examination of selected expressed sequence tags also showed an overall decrease in mRNA transcription levels in samples derived from anoxic nuclei as compared with normoxic nuclei. Control of ribosomal translation was also examined by assessing the levels of the eukaryotic initiation factors eIF-2α and eIF-4E and the eukaryotic elongation factor-1γ (eEF-1γ). Levels of eIF-4E and eEF-1γ did not change between aerobic and anoxic states, but the amount of phosphorylated inactive eIF-2α rose strongly under anoxic conditions indicating that control of this factor is key to suppressing protein translation in anoxic snails. Since gene transcription is an ATP expensive process in cells, suppression of transcription to minimum levels provides substantial energy savings for the hepatopancreas, and the organism as a whole, under anoxic conditions.
Starting Page	243
Ending Page	249
Page Count	7
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	303
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Springer US
Publisher Date	2007-05-15
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Littorina littorea Snail Transcription Metabolic suppression Anoxia Mollusc Cardiology Oncology Medical Biochemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Clinical Biochemistry Molecular Biology

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