NDLI: Calorimetric and spectroscopic binding studies of amoxicillin with human serum albumin

Content Provider	Springer Nature Link
Author	Yasmeen, Shama Rabbani, Gulam
Copyright Year	2016
Abstract	Amoxicillin is a common antibiotic drug used for preventing bacterial colonization of urinary and skin infections. The binding of amoxicillin (AX) to human serum albumin (HSA) in sodium phosphate buffer solution at pH 7.4 has been investigated by UV–visible spectroscopy, fluorescence spectroscopy, Förster resonance energy transfer, isothermal titration calorimetry (ITC), circular dichroism (CD) and FTIR spectroscopy. The binding studies using ITC revealed that the interaction is entropy driven rather than enthalpy as the change in enthalpy (ΔH°) and change in entropy (TΔS°) both are positive, indicating the endothermic reaction with low affinity and hydrophobic interaction. UV–visible absorption spectra of HSA are increased upon addition of AX which shows complex formation of HSA–AX. The fluorescence spectra reveal that the AX has an ability to quench the intrinsic fluorescence of HSA tryptophan through a static quenching procedure. The molecular distance r between donor (HSA) and acceptor (AX) was estimated according to Förster theory of nonradiative energy transfer. The CD spectra showed that the α-helix of HSA increases with increasing the amount of AX. The thermal denaturation has been studied by far-UV CD, and it is found that HSA stability is decreased by the complex formation of HSA and AX. The remarkable change in amide I peak position in infrared spectrum after interaction with AX indicated that secondary structure of HSA has been changed.
Starting Page	1445
Ending Page	1455
Page Count	11
File Format	PDF
ISSN	13886150
Journal	Journal of thermal analysis
Volume Number	127
Issue Number	2
e-ISSN	15882926
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-06-14
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amoxicillin Circular dichroism Fluorescence spectroscopy Human serum albumin Isothermal titration calorimetry UV–visible spectroscopy Physical Chemistry Analytical Chemistry Polymer Sciences Inorganic Chemistry Measurement Science and Instrumentation
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry Condensed Matter Physics

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