NDLI: Rhodamine B nanocrystals: elaborations, characterizations and functionalizations for biosensing applications

Content Provider	Springer Nature Link
Author	Dubuisson, Emilie Marty, Laëtitia Cattoën, Xavier Pansu, Robert Szunerits, Sabine Ibanez, Alain
Copyright Year	2014
Abstract	A biological sensor based on fluorescent organic nanocrystals (NCs) of Rhodamine B grown in sol–gel thin films was developed. The original signalization function is based on fluorescence contrasts of NCs, which exhibit a simple fluorescence signature, good photostability and higher fluorescence intensities compared to dispersed dye molecules. Thanks to a well-controlled dissolution process of the sol–gel surface, accurately followed by atomic force microscopy, the NCs were made emerging just a few nanometers above the silicate thin films to be directly accessible to biological macromolecules. Thus, hairpin-shaped DNA, functionalized by a probe-molecule (DNA probe), has been grafted onto nanocrystal surfaces leading to a fluorescence quenching by Forster resonance energy transfer. After hybridization of these hairpin-shaped DNA probes with their complementary DNA-target, the molecular probes and NCs are pulled apart, stopping thus the quenching. This “turn-on” of nanocrystal fluorescence allows thus a label-free DNA detection. The preparation methodology of the signalization function, its functionalization by hairpin-shaped DNA probes and first DNA-sensor experiments are presented.
Starting Page	179
Ending Page	188
Page Count	10
File Format	PDF
ISSN	09280707
Journal	Journal of Sol-Gel Science and Technology
Volume Number	72
Issue Number	1
e-ISSN	15734846
Language	English
Publisher	Springer US
Publisher Date	2014-07-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Organic nanocrystals Sol–gel Thin film Confined nucleation Fluorescence Biological sensors Fluorescence quenching Surface functionalization Ceramics, Glass, Composites, Natural Methods Inorganic Chemistry Optical and Electronic Materials Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Chemistry Materials Chemistry Biomaterials Condensed Matter Physics Electronic, Optical and Magnetic Materials

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Fluorescent nanocrystals grown in sol–gel thin films for generic stable and sensitive sensors

Fluorescence spectroscopic study of dip coated sol-gel thin film internal environment using fluorescent probes Hoechst33258 and Pyranine

Stress evolution on gel-to-ceramic thin film conversion

Photo-quenched luminescence in Co(II)-doped sol–gel zirconia

Deposition of silica thin films formed by sol–gel method

Fluorescent organic nanocrystal confined in sol–gel matrix for bio-imaging

Structural evolution of nanostructured barium titanate thin film sol–gel derived

Indium selenide thin film preparation by sol–gel technique

Sol–gel and transfer technique for fabricating dual ceramic thin film patterns on plastics

Rhodamine B nanocrystals: elaborations, characterizations and functionalizations for biosensing applications

Similar Documents

Fluorescent nanocrystals grown in sol–gel thin films for generic stable and sensitive sensors

Fluorescence spectroscopic study of dip coated sol-gel thin film internal environment using fluorescent probes Hoechst33258 and Pyranine

Stress evolution on gel-to-ceramic thin film conversion

Photo-quenched luminescence in Co(II)-doped sol–gel zirconia

Deposition of silica thin films formed by sol–gel method

Fluorescent organic nanocrystal confined in sol–gel matrix for bio-imaging

Structural evolution of nanostructured barium titanate thin film sol–gel derived

Indium selenide thin film preparation by sol–gel technique

Sol–gel and transfer technique for fabricating dual ceramic thin film patterns on plastics

Rhodamine B nanocrystals: elaborations, characterizations and functionalizations for biosensing applications