NDLI: Thermodynamical Studies of an Example Peptide Containing Metaaminobenzoic Acid (MABA) that Promotes Bends in Proteins

Content Provider	Springer Nature Link
Author	Makowska, Joanna Uber, Dorota Chmurzyński, Lech
Copyright Year	2015
Abstract	The pK $_{a}$ values of the peptide derived from the immunoglobulin binding protein G, Ac-Asp-Asp-Ala-Thr-Lys-Thr-NH$_{2}$ (Dag1), and its derivative Ac-Asp-Asp-(meta-aminobenzoic acid)-Lys-Thr-NH$_{2}$ (Dag1_M), were measured using potentiometric titrations in the temperature range from 25 to 50 °C. The heat capacity profiles for both systems were determined by differential scanning calorimetry in the temperature range 10–90 °C and CD measurements were made at nine different temperatures.To decrease the mobility of the peptide under study (Dag1), and to ensure a better stability of its structure (better stiffness), the amino acids from the turn region of Dag1 (Ala3 and Thr4) in the original sequences were replaced by the meta-aminobenzoic acid (MABA) residue, which acts as a β-mimetic. The results obtained in this work suggest that the charged residues present in both sequences (Asp1, Asp2 and Lys5) are in similar environments in both peptides. Therefore, the shapes of the dominant conformations of Dag1 and Dag1_M should be similar to each other; specifically, both peptides probably have a bent structure. Negative bands in the CD spectra are present at 195 and 215 nm for Dag1 and Dag1_M, respectively, which are characteristic of a statistical coil conformation. The pK $_{a}$ values for charged amino acids (two Asp and one Lys) at t = 25 °C in both sequences are very similar, which suggests that the environments for those ionizable groups are similar.
Starting Page	223
Ending Page	236
Page Count	14
File Format	PDF
ISSN	00959782
Journal	Journal of Solution Chemistry
Volume Number	44
Issue Number	2
e-ISSN	15728927
Language	English
Publisher	Springer US
Publisher Date	2015-02-21
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Potentiometric titration β-turn pK $_{a}$ values Early stages of protein folding Physical Chemistry Industrial Chemistry/Chemical Engineering Geochemistry Oceanography Inorganic Chemistry Condensed Matter Physics
Content Type	Text
Resource Type	Article
Subject	Biochemistry Molecular Biology Biophysics Physical and Theoretical Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

PK $_{a}$ Determinations for Montelukast Sodium and Levodropropizine

Investigation of the Role of Ionic Liquids in Tuning the pK $_{a}$ Values of Some Anionic Indicators

Acidity Constants of Supported Salts of Heteropoly Acids Using a Methodology Related to the Potentiometric Mass Titration Technique

Determination of pK $_{aIL}$ Values of Three Chelating Extractants in ILs: Consequences for the Extraction of 4f Elements

UV Spectroscopic Method for Determining pK $_{a}$ Values of Some Antipsychotic Drugs in Water and Acetonitrile–Water Binary Mixtures

Modified Screen-Printed Electrode for Potentiometric Determination of Copper(II) in Water Samples

PK $_{a}$ Constant Determination of Two Triazole Pesticides: Tebuconazole and Penconazole

Mixed-Ligand Complexes of 2-(Aminomethyl)benzimidazole Palladium(II) with Various Biologically Relevant Ligands

Dissociation Constant of Acetic Acid in (N,N-Dimethylformamide + Water) Mixtures at the Temperature 298.15 K

Thermodynamical Studies of an Example Peptide Containing Metaaminobenzoic Acid (MABA) that Promotes Bends in Proteins

Similar Documents

PK $_{a}$ Determinations for Montelukast Sodium and Levodropropizine

Investigation of the Role of Ionic Liquids in Tuning the pK $_{a}$ Values of Some Anionic Indicators

Acidity Constants of Supported Salts of Heteropoly Acids Using a Methodology Related to the Potentiometric Mass Titration Technique

Determination of pK $_{aIL}$ Values of Three Chelating Extractants in ILs: Consequences for the Extraction of 4f Elements

UV Spectroscopic Method for Determining pK $_{a}$ Values of Some Antipsychotic Drugs in Water and Acetonitrile–Water Binary Mixtures

Modified Screen-Printed Electrode for Potentiometric Determination of Copper(II) in Water Samples

PK $_{a}$ Constant Determination of Two Triazole Pesticides: Tebuconazole and Penconazole

Mixed-Ligand Complexes of 2-(Aminomethyl)benzimidazole Palladium(II) with Various Biologically Relevant Ligands

Dissociation Constant of Acetic Acid in (N,N-Dimethylformamide + Water) Mixtures at the Temperature 298.15 K

Thermodynamical Studies of an Example Peptide Containing Metaaminobenzoic Acid (MABA) that Promotes Bends in Proteins