NDLI: Acid activated montmorillonite: an efficient immobilization support for improving reusability, storage stability and operational stability of enzymes

Content Provider	Springer Nature Link
Author	Sanjay, Gopinath Sugunan, Sankaran
Copyright Year	2007
Abstract	Three enzymes, α-amylase, glucoamylase and invertase, were immobilized on acid activated montmorillonite K 10 via two independent techniques, adsorption and covalent binding. The immobilized enzymes were characterized by XRD, N$_{2}$ adsorption measurements and $^{27}$Al MAS-NMR spectroscopy. The XRD patterns showed that all enzymes were intercalated into the clay inter-layer space. The entire protein backbone was situated at the periphery of the clay matrix. Intercalation occurred through the side chains of the amino acid residues. A decrease in surface area and pore volume upon immobilization supported this observation. The extent of intercalation was greater for the covalently bound systems. NMR data showed that tetrahedral Al species were involved during enzyme adsorption whereas octahedral Al was involved during covalent binding. The immobilized enzymes demonstrated enhanced storage stability. While the free enzymes lost all activity within a period of 10 days, the immobilized forms retained appreciable activity even after 30 days of storage. Reusability also improved upon immobilization. Here again, covalently bound enzymes exhibited better characteristics than their adsorbed counterparts. The immobilized enzymes could be successfully used continuously in the packed bed reactor for about 96 hours without much loss in activity. Immobilized glucoamylase demonstrated the best results.
Starting Page	359
Ending Page	367
Page Count	9
File Format	PDF
ISSN	13802224
Journal	Journal of Porous Materials
Volume Number	15
Issue Number	3
e-ISSN	15734854
Language	English
Publisher	Springer US
Publisher Date	2007-03-29
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	α-Amylase Glucoamylase Invertase Immobilization Montmorillonite Adsorption Covalent binding Physical Chemistry Characterization and Evaluation of Materials Catalysis
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Materials Science Mechanical Engineering

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