NDLI: Analysis of Cytosolic pH Changes in Thymocytes During Early Apoptosis with Improved Three-Channel Real-Time Fluorescence Imaging

Content Provider	Springer Nature Link
Author	Shu, Zhang Xiaochen, Liu Chuyun, Deng Man, Han Juhua, Pan Xinghua, Liao Xin, Qi Shaojin, Duan Wanyun, Ma
Copyright Year	2014
Abstract	Cytosolic pH changes in single thymocytes in the early stages of apoptosis were examined with spectral-crosstalk-improved three-channel real-time fluorescence imaging. Spectral crosstalk was greatly reduced by this improved image processing method. Compared with normal thymocytes, the improved image processing method showed that the cytosolic pH value of thymocytes undergoing apoptosis was lower. A slight diversity in the intracellular pH values of samples subjected to different drug treatments was also observed. This new image processing method is beneficial to the application of the three-channel real-time fluorescence imaging to biological processes.
Starting Page	1055
Ending Page	1059
Page Count	5
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	24
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2014-04-17
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Three-channel Spectral crosstalk Real-time fluorescence imaging Apoptosis Cytosolic pH Biomedicine general Biophysics and Biological Physics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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Analysis of Cytosolic pH Changes in Thymocytes During Early Apoptosis with Improved Three-Channel Real-Time Fluorescence Imaging