NDLI: Stabilization of NADH-dehydrogenase in Mitochondria by Guanosine Phosphates and Adenosine Phosphates

Content Provider	Springer Nature Link
Author	Frolova, M. S. Vekshin, N. L.
Copyright Year	2014
Abstract	It is known that one of the reasons, leading to the development of neuromuscular diseases, including Parkinson’s disease, is damage of the mitochondrial NADH-dehydrogenase. Perhaps, it happens when NADH-dehydrogenase loses connection with its coenzyme – flavine mononucleotide (FMN) that occurs at various influences on the enzyme. Previously, we have developed a method, based on fluorescence spectroscopy, to monitor the rate of exit of FMN from isolated mitochondria to solution. Also, we obtained the data that this process is blocked by the enzyme substrate – NADH or by the product – NAD. Recently, we found that this process is strongly blocked by adenine analogs of NAD, contained phosphates: ATP, ADP, and AMP. Adenosine phosphates are able to stabilize the FMN molecule in NADH-dehydrogenase. Using fluorescence spectroscopy and photocolorimetry, we have tested also other natural purine compounds - cAMP, cGMP, GMP, GDP, GTP, IMP, inosine, guanine, and caffeine. It is found that such derivatives of guanine as GMP, GDP, and GTP can prevent the release of FMN into solution. Guanine, cGMP, cAMP and caffeine did not prevent this process. The obtained data allow understand the mechanism of mitochondrial diseases, involving damage of mitochondrial NADH-dehydrogenase, and may help in development of medicines for treatment of these diseases.
Starting Page	1061
Ending Page	1066
Page Count	6
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	24
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2014-04-11
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	NADH-dehydrogenase Mitochondria Neuromuscular diseases Parkinson’s disease Mitochondrial diseases Guanosine phosphates FMN Fluorescence spectroscopy Flavine fluorescence Biomedicine general Biophysics and Biological Physics Biotechnology Biochemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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