NDLI: Fluorescence Imaging of Stem Cells, Cancer Cells and Semi-Thin Sections of Tissues using Silica-Coated CdSe Quantum Dots

Content Provider	Springer Nature Link
Author	Vibin, M. Vinayakan, R. John, Annie Raji, V. Rejiya, C. S. Abraham, Annie
Copyright Year	2011
Abstract	Trioctylphosphine oxide capped cadmium selenide quantum dots, synthesized in organic media were rendered water soluble by silica overcoating. Silanisation was done by a simple reverse microemulsion method using aminopropyl silane as the silica precursor. Further, the strong photoluminescence of the silica-coated CdSe quantum dots has been utilized to visualize rabbit adipose tissue-derived mesenchymal stem cells (RADMSCs) and Daltons lymphoma ascites (DLA) cancerous cells in vitro. Subsequently the in vivo fluorescence behaviours of QDs in the tissues were also demonstrated by intravenous administration of the QDs in Swiss albino mice. The fluorescence microscopic images in the stem cells, cancer cells and semi-thin sections of mice organs proved the strong luminescence property of silica-coated quantum dots under biological systems. These results establish silica-coated CdSe QDs as extremely useful tools for molecular imaging and cell tracking to study the cell division and metastasis of cancer and other diseases.
Starting Page	1365
Ending Page	1370
Page Count	6
File Format	PDF
ISSN	10530509
Journal	Journal of Fluorescence
Volume Number	21
Issue Number	4
e-ISSN	15734994
Language	English
Publisher	Springer US
Publisher Date	2011-01-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DLA cells Luminescence Quantum dots RADMSCs Semi-thin section Biochemistry Biotechnology Biophysics and Biological Physics Biomedicine general Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Sociology and Political Science Spectroscopy Law Biochemistry Clinical Biochemistry Clinical Psychology

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